Watanabe T, Shinohara N, Sazawa A, Harabayashi T, Ogiso Y, Koyanagi T, Takiguchi M, Hashimoto A, Kuzumaki N, Yamashita M, Tanaka M, Grossman H B, Benedict W F
'Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Cancer Gene Ther. 2000 Dec;7(12):1575-80. doi: 10.1038/sj.cgt.7700261.
Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU-7 and UM-UC-2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant beta-galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer.
将人膀胱癌细胞原位植入免疫缺陷小鼠体内是研究膀胱癌生物学特性及治疗效果的重要工具。然而，经尿道向鼠膀胱内灌注人膀胱癌细胞后，肿瘤植入和生长的发生率一直较低或难以重复。不过，我们采用改良的膀胱内技术和人膀胱癌细胞系KU-7及UM-UC-2，成功获得了高发生率且可重复的浅表性膀胱肿瘤。此外，膀胱内给予LacZ腺病毒载体后，这些膀胱肿瘤以及正常尿路上皮中均出现了显著的β-半乳糖苷酶表达，这与糖胺聚糖层的去除有关。由于这种改良技术能产生高发生率的浅表性人类肿瘤生长，并可评估基因转移的效果，因此它应是研究人膀胱癌膀胱内基因治疗的有用模型。
