Raven Peter A, D'Costa Ninadh M, Moskalev Igor, Tan Zheng, Frees Sebastian, Chavez-Munoz Claudia, So Alan I
Vancouver Prostate Centre, Department of Urologic Sciences, University of British Columbia Vancouver, BC, Canada.
Universitätsmedizin der Johannes Gutenberg-Universität Mainz Mainz, Germany.
Am J Clin Exp Urol. 2018 Dec 20;6(6):245-259. eCollection 2018.
We have developed a murine intravesical orthotopic human bladder cancer (mio-hBC) model for the establishment of superficial urothelial cell carcinomas. In this model we catheterize female atyhmic nude mice and pre-treat the bladder with poly-L-lysine for 15 minutes, followed by intravesical instillation of luciferase-transfected human UM-UC-3 cells. Cancer cells are quantified by bioluminescent imaging which has been validated by small animal ultrasound. Poly-L-lysine pre-treatment increased engraftment rate (84.4%) and resulted in faster growing tumors than trypsin pre-treatment. In addition, tumors respond through a decrease in growth and increase in apoptosis to chemotherapy with mitomycin C. Previous intravesical models utilized KU7 cells which have been later determined to be of non-bladder origin. They display markers consistent with HeLa cells, requiring a need for a true intravesical bladder model. Efficient engraftment and rapid superficial growth patterning of the human bladder tumor differentiate this in vivo orthotopic model from previous bladder models.
我们已经开发出一种用于建立浅表性尿路上皮细胞癌的小鼠膀胱原位人膀胱癌（mio-hBC）模型。在该模型中，我们将雌性无胸腺裸鼠进行导尿，并使用聚-L-赖氨酸对膀胱进行15分钟的预处理，随后膀胱内灌注荧光素酶转染的人UM-UC-3细胞。癌细胞通过生物发光成像进行定量，该成像已通过小动物超声验证。聚-L-赖氨酸预处理提高了植入率（84.4%），并且与胰蛋白酶预处理相比，肿瘤生长更快。此外，肿瘤对丝裂霉素C化疗的反应是生长减缓且凋亡增加。以前的膀胱内模型使用的KU7细胞后来被确定并非源自膀胱。它们显示出与HeLa细胞一致的标志物，因此需要一个真正的膀胱内膀胱模型。人膀胱肿瘤的高效植入和快速浅表生长模式使这种体内原位模型有别于先前的膀胱模型。
