Craig R D
Eur J Clin Invest. 1975 Feb;5(1):69-74. doi: 10.1111/j.1365-2362.1975.tb00430.x.
A comparison of the rates of synthesis of collagen in normal skin, normal and hypertrophic scars, and keloids has been made by measuring the rate of incorporation of [14-C]-proline into peptide-bound [14-C]-hydroxyproline by tissue minces in vitro. The rate of synthesis of collagen, as measured by this technique, was significantly higher in skin than in normal scars whether the incorporation of radioactivity into hydroxyproline were expressed in terms of wet weight of tissue, weight of tissue DNA or weight of tissue hydroxyproline. The abnormal scar types exhibited similar rates of collagen synthesis, which were significantly higher than the rate in normal scars. Although the rates in both abnormal scar types appeared to be similar to that in normal skin when expressed in terms of wet weight of tissue, and weight of tissue hydroxyproline, they were seen to be lower than in skin in terms of weight of tissue DNA. The rate of synthesis of proteins generally, as measured by total radioactivity in non-diffusible peptides, was highest in normal skin and hypertrophic scar and lowest in keloid. The ratio of radioactivity in non-diffusible hydroxyproline to total non-diffusible radioactivity was almost twice as high in keloid as in normal scar, with intermediate values being observed in hypertrophic scar and normal skin. This indicated that collagen accounted for a higher proportion of the proteins being synthesised in keloid than in normal scar. The results confirm previous conclusions, from determination of the activity of the enzyme collagen proline hydroxylase, that the excessive accumulation of collagen in hypertrophic scars and keloids may, at least in part, be due to abnormally high rates of collagen synthesis in comparison to normal scars.
通过在体外测量组织碎块中[14-C]-脯氨酸掺入肽结合的[14-C]-羟脯氨酸的速率，对正常皮肤、正常瘢痕和肥厚性瘢痕以及瘢痕疙瘩中胶原蛋白的合成速率进行了比较。用该技术测量，无论放射性掺入羟脯氨酸是以组织湿重、组织DNA重量还是组织羟脯氨酸重量来表示，皮肤中胶原蛋白的合成速率均显著高于正常瘢痕。异常瘢痕类型表现出相似的胶原蛋白合成速率，显著高于正常瘢痕中的合成速率。尽管以组织湿重和组织羟脯氨酸重量表示时，两种异常瘢痕类型的合成速率似乎与正常皮肤相似，但以组织DNA重量表示时，它们低于皮肤中的合成速率。一般来说，用不可扩散肽中的总放射性测量，正常皮肤和肥厚性瘢痕中蛋白质的合成速率最高，瘢痕疙瘩中最低。不可扩散羟脯氨酸中的放射性与总不可扩散放射性的比值在瘢痕疙瘩中几乎是正常瘢痕中的两倍，在肥厚性瘢痕和正常皮肤中观察到的是中间值。这表明与正常瘢痕相比，瘢痕疙瘩中合成的蛋白质中胶原蛋白所占比例更高。这些结果证实了先前从测定胶原脯氨酸羟化酶活性得出的结论，即肥厚性瘢痕和瘢痕疙瘩中胶原蛋白的过度积累至少部分可能是由于与正常瘢痕相比胶原蛋白合成速率异常高。
