McCoy B J, Galdun J, Cohen I K
In Vitro. 1982 Jan;18(1):79-86. doi: 10.1007/BF02796388.
Cell proliferation and collagen synthesis were analyzed in high, medium, and low density keloid and normal skin fibroblasts and also at various times during the course of in vitro aging to expand previous findings of increased collagen synthesis in keloid compared to normal skin fibroblasts. It was found that both keloid and normal fibroblasts (less than 20 population doublings) responded similarly to high, medium, and low initial plating densities; however, sparsely plated keloid fibroblasts exhibited a loss of replicative capacity earlier in their in vitro lifespan than did sparsely plated normal skin fibroblasts. When analyzed at population doubling levels 2 to 38, collagen synthesis was elevated in keloid compared to normal skin fibroblasts but decreased at the same rate in both cell types throughout this in vitro interval.
在高密度、中密度和低密度瘢痕疙瘩及正常皮肤成纤维细胞中分析了细胞增殖和胶原蛋白合成，并且在体外老化过程中的不同时间点进行了分析，以扩展先前关于瘢痕疙瘩中胶原蛋白合成比正常皮肤成纤维细胞增加的研究结果。结果发现，瘢痕疙瘩和成纤维细胞（少于20次群体倍增）对高、中、低初始接种密度的反应相似；然而，稀疏接种的瘢痕疙瘩成纤维细胞在其体外寿命中比稀疏接种的正常皮肤成纤维细胞更早地表现出复制能力丧失。在群体倍增水平2至38进行分析时，瘢痕疙瘩中的胶原蛋白合成比正常皮肤成纤维细胞升高，但在整个体外培养期间，两种细胞类型的胶原蛋白合成均以相同速率下降。
