海洋沉积物中病毒丰度的测定。
Determination of virus abundance in marine sediments.
作者信息
Danovaro R, Dell'Anno A, Trucco A, Serresi M, Vanucci S
机构信息
Institute of Marine Science, University of Ancona, 60131 Ancona, Italy.
出版信息
Appl Environ Microbiol. 2001 Mar;67(3):1384-7. doi: 10.1128/AEM.67.3.1384-1387.2001.
Abstract
In this study, we optimized procedures to enumerate viruses from marine sediments by epifluorescence microscopy using SYBR Green I as a stain. The highest virus yields from the bulk of the sediments were obtained by utilizing pyrophosphate and 3 min of sonication. The efficiency of extraction benthic viruses by pyrophosphate-ultrasound treatment was about 60% of the extractable virus particles. Samples treated with nucleases had increased virus counts, suggesting a masking effect of extracellular DNA. No significant differences were observed between virus counts obtained by epifluorescence microscopy and transmission electron microscopy. Both formaldehyde and glutaraldehyde gave significant reductions of virus counts after only 24 h of sediment storage, but no further loss occurred after 7 days.
摘要
在本研究中，我们优化了通过落射荧光显微镜使用SYBR Green I作为染色剂从海洋沉积物中计数病毒的程序。通过利用焦磷酸盐和3分钟的超声处理，从大部分沉积物中获得了最高的病毒产量。焦磷酸盐-超声处理提取底栖病毒的效率约为可提取病毒颗粒的60%。用核酸酶处理的样品病毒计数增加，表明细胞外DNA具有掩盖作用。落射荧光显微镜和透射电子显微镜获得的病毒计数之间未观察到显著差异。甲醛和戊二醛在沉积物储存仅24小时后均使病毒计数显著降低，但7天后未发生进一步损失。
相似文献
1
Determination of virus abundance in marine sediments.海洋沉积物中病毒丰度的测定。
Appl Environ Microbiol. 2001 Mar;67(3):1384-7. doi: 10.1128/AEM.67.3.1384-1387.2001.
2
Flow cytometric analysis of bacteria- and virus-like particles in lake sediments.湖泊沉积物中细菌和病毒样颗粒的流式细胞术分析
J Microbiol Methods. 2006 Mar;64(3):316-32. doi: 10.1016/j.mimet.2005.05.008. Epub 2005 Aug 2.
3
Assessment of factors influencing direct enumeration of viruses within estuarine sediments.河口沉积物中病毒直接计数的影响因素评估
Appl Environ Microbiol. 2006 Jul;72(7):4767-74. doi: 10.1128/AEM.00297-06.
4
Determination of virus abundance, diversity and distribution in a municipal wastewater treatment plant.城市污水处理厂中病毒丰度、多样性及分布的测定
Water Res. 2009 Mar;43(4):1101-9. doi: 10.1016/j.watres.2008.11.039. Epub 2008 Dec 6.
5
Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold.应用数字图像分析和流式细胞术对用SYBR金染色的海洋病毒进行计数
Appl Environ Microbiol. 2001 Feb;67(2):539-45. doi: 10.1128/AEM.67.2.539-545.2001.
6
Viruses, prokaryotes and DNA in the sediments of a deep-hypersaline anoxic basin (DHAB) of the Mediterranean Sea.地中海深海高盐缺氧盆地（DHAB）沉积物中的病毒、原核生物和DNA。
Environ Microbiol. 2005 Apr;7(4):586-92. doi: 10.1111/j.1462-2920.2005.00727.x.
7
Determination of virus abundance by epifluorescence microscopy.通过落射荧光显微镜法测定病毒丰度。
Methods Mol Biol. 2009;501:87-95. doi: 10.1007/978-1-60327-164-6_10.
8
An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry.通过流式细胞术定量海洋沉积物病毒的高级方案。
Viruses. 2021 Jan 13;13(1):102. doi: 10.3390/v13010102.
9
Techniques for the recovery of enteric viruses from artificially contaminated marine sediments.从人工污染的海洋沉积物中回收肠道病毒的技术。
Ig Sanita Pubbl. 2007 Jul-Aug;63(4):419-31.
10
Study of changes in bacterial and viral abundance in formaldehyde-fixed water samples by epifluorescence microscopy.运用荧光显微镜研究甲醛固定水样中细菌和病毒丰度的变化。
Environ Monit Assess. 2011 Jun;177(1-4):227-31. doi: 10.1007/s10661-010-1629-7. Epub 2010 Jul 28.
引用本文的文献
1
Phages Exist as Episomal Pseudolysogens Across Diverse Soil Bacteria.噬菌体以附加型假溶原菌的形式存在于多种土壤细菌中。
Microorganisms. 2025 May 28;13(6):1239. doi: 10.3390/microorganisms13061239.
2
Review of Methods for Studying Viruses in the Environment and Organisms.环境与生物中病毒研究方法综述
Viruses. 2025 Jan 11;17(1):86. doi: 10.3390/v17010086.
3
Evaluating storage conditions and enhancement strategies on viral biomarker recovery for WBE applications.评估储存条件和增强策略对 WBE 应用中病毒生物标志物回收的影响。
Water Sci Technol. 2024 Jul;90(1):213-224. doi: 10.2166/wst.2024.203. Epub 2024 Jun 13.
4
Community ecology and functional potential of bacteria, archaea, eukarya and viruses in Guerrero Negro microbial mat.格雷罗内格罗微生物席中的细菌、古菌、真核生物和病毒的群落生态学和功能潜力。
Sci Rep. 2024 Jan 31;14(1):2561. doi: 10.1038/s41598-024-52626-y.
5
Improved virus concentration methods for wash waters from decontamination of permeable and non-permeable surfaces.改进的病毒浓缩方法，用于去污可渗透和不可渗透表面的洗涤水。
J Virol Methods. 2023 Dec;322:114826. doi: 10.1016/j.jviromet.2023.114826. Epub 2023 Sep 29.
6
CRISPR-Cas9 Based Bacteriophage Genome Editing.基于 CRISPR-Cas9 的噬菌体基因组编辑。
Microbiol Spectr. 2022 Aug 31;10(4):e0082022. doi: 10.1128/spectrum.00820-22. Epub 2022 Jul 26.
7
Imaging Techniques for Detecting Prokaryotic Viruses in Environmental Samples.用于检测环境样本中原核病毒的成像技术。
Viruses. 2021 Oct 21;13(11):2126. doi: 10.3390/v13112126.
8
Bacteriophages in Biological Wastewater Treatment Systems: Occurrence, Characterization, and Function.生物废水处理系统中的噬菌体：存在、表征及功能
Front Microbiol. 2021 Sep 30;12:730071. doi: 10.3389/fmicb.2021.730071. eCollection 2021.
9
An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry.通过流式细胞术定量海洋沉积物病毒的高级方案。
Viruses. 2021 Jan 13;13(1):102. doi: 10.3390/v13010102.
10
New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments.海洋沉积物中病毒与原核生物比例（VPR）的新见解
Front Microbiol. 2020 May 29;11:1102. doi: 10.3389/fmicb.2020.01102. eCollection 2020.
本文引用的文献
1
High concentrations of viruses in the sediments of Lac Gilbert, Québec.在魁北克吉尔伯特湖的沉积物中发现高浓度病毒。
Microb Ecol. 1996 Mar;31(2):141-51. doi: 10.1007/BF00167860.
2
Effects of viruses on nutrient turnover and growth efficiency of noninfected marine bacterioplankton.病毒对非感染海洋细菌浮游生物养分周转率和生长效率的影响。
Appl Environ Microbiol. 1996 Jun;62(6):1991-7. doi: 10.1128/aem.62.6.1991-1997.1996.
3
Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy.海水中病毒丰度的评估：荧光显微镜和透射电子显微镜法。
Appl Environ Microbiol. 1991 Sep;57(9):2731-4. doi: 10.1128/aem.57.9.2731-2734.1991.
4
Viral density and virus-to-bacterium ratio in deep-sea sediments of the Eastern Mediterranean.东地中海深海沉积物中的病毒密度和病毒与细菌的比率。
Appl Environ Microbiol. 2000 May;66(5):1857-61. doi: 10.1128/AEM.66.5.1857-1861.2000.
5
Pelagic-benthic coupling of nucleic acids in an abyssal location of the northeastern Atlantic Ocean.东北大西洋一个深海区域中核酸的远洋-底栖耦合
Appl Environ Microbiol. 1999 Oct;65(10):4451-7. doi: 10.1128/AEM.65.10.4451-4457.1999.
6
Marine viruses and their biogeochemical and ecological effects.海洋病毒及其生物地球化学和生态效应。
Nature. 1999 Jun 10;399(6736):541-8. doi: 10.1038/21119.
7
Enumeration of marine viruses in culture and natural samples by flow cytometry.通过流式细胞术对培养物和自然样本中的海洋病毒进行计数。
Appl Environ Microbiol. 1999 Jan;65(1):45-52. doi: 10.1128/AEM.65.1.45-52.1999.
8
Nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments: comparison of spectrophotometric, fluorometric, and HighPerformance liquid chromatography methods and estimation of detrital DNA.海洋沉积物中核酸（DNA、RNA）定量及RNA/DNA比率测定：分光光度法、荧光法和高效液相色谱法的比较以及碎屑DNA的估算
Appl Environ Microbiol. 1998 Sep;64(9):3238-45. doi: 10.1128/AEM.64.9.3238-3245.1998.
9
Distribution of viral abundance in the reef environment of Key Largo, Florida.佛罗里达州基拉戈礁环境中病毒丰度的分布情况。
Appl Environ Microbiol. 1993 Mar;59(3):718-24. doi: 10.1128/aem.59.3.718-724.1993.
10
High abundance of viruses found in aquatic environments.在水生环境中发现大量病毒。
Nature. 1989 Aug 10;340(6233):467-8. doi: 10.1038/340467a0.
Zotero 插件