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Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction.血液的采样与储存以及通过聚合酶链反应检测疟原虫
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Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers.基于比色PCR法诊断旅行者恶性疟原虫疟疾的评估
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Comparison of the ParaSight-F test and the ICT Malaria Pf test with the polymerase chain reaction for the diagnosis of Plasmodium falciparum malaria in travellers.ParaSight-F检测和ICT疟疾Pf检测与聚合酶链反应在诊断旅行者恶性疟原虫疟疾中的比较。
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将IsoCode STIX和FTA基因保护采集基质作为用于通过聚合酶链反应（PCR）诊断疟疾的全血储存和处理装置进行比较。

Comparison of IsoCode STIX and FTA Gene Guard collection matrices as whole-blood storage and processing devices for diagnosis of malaria by PCR.

作者信息

Zhong K J, Salas C J, Shafer R, Gubanov A, Gasser R A, Magill A J, Forney J R, Kain K C

机构信息

Centre for Travel and Tropical Medicine, Division of Infectious Diseases, Department of Medicine, Toronto General Hospital and the University of Toronto, 200 Elizabeth St., M5G 2C4 Toronto, Ontario, Canada.

出版信息

J Clin Microbiol. 2001 Mar;39(3):1195-6. doi: 10.1128/JCM.39.3.1195-1196.2001.

DOI:10.1128/JCM.39.3.1195-1196.2001
PMID:11230458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87904/
Abstract

We compared two collection devices, IsoCode and FTA, with whole blood for the diagnosis of malaria by PCR (n = 100). Using whole blood as the reference standard, both devices were sensitive for the detection of single-species malaria infections by PCR (> or =96%). However, the detection of mixed infections was suboptimal (IsoCode was 42% sensitive, and FTA was 63% sensitive).

摘要

我们比较了两种采集设备IsoCode和FTA，使用全血样本通过聚合酶链反应（PCR）诊断疟疾（n = 100）。以全血作为参考标准，两种设备通过PCR检测单一物种疟疾感染时均具有较高敏感性（≥96%）。然而，对于混合感染的检测效果欠佳（IsoCode敏感性为42%，FTA敏感性为63%）。