Yoshida S, Ito M, Nishida I, Watanabe A
Department of Biological Sciences, Graduated School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan.
Plant Cell Physiol. 2001 Feb;42(2):170-8. doi: 10.1093/pcp/pce021.
Nine cDNAs, representing genes in which the transcripts accumulated in senescent leaves of Arabidopsis thaliana, were isolated by differential display reverse transcription polymerase chain reaction (DDRT-PCR) and the genes were designated yellow-leaf-specific gene 1 to 9 (YLS1-YLS9). Sequence analysis revealed that none of the YLS genes, except YLS6, had been reported as senescence-up-regulated genes. RNA gel blot analysis revealed that the transcripts of YLS3 accumulated at the highest level at an early senescence stage, whereas the transcripts from the other YLS genes reached their maximum levels in late senescence stages. Transcripts of YLS genes showed various accumulation patterns under natural senescence, and under artificial senescence induced by darkness, ethylene or ABA. These expression characteristics of YLS genes will be useful as potential molecular markers, which will enhance our understanding of natural and artificial senescence processes.
通过差异显示逆转录聚合酶链反应（DDRT-PCR）分离出9个cDNA，它们代表在拟南芥衰老叶片中转录本积累的基因，这些基因被命名为黄叶特异性基因1至9（YLS1-YLS9）。序列分析表明，除YLS6外，没有一个YLS基因被报道为衰老上调基因。RNA凝胶印迹分析显示，YLS3的转录本在衰老早期积累水平最高，而其他YLS基因的转录本在衰老后期达到最高水平。YLS基因的转录本在自然衰老以及黑暗、乙烯或脱落酸诱导的人工衰老条件下表现出各种积累模式。YLS基因的这些表达特征将作为潜在的分子标记，有助于我们更好地理解自然和人工衰老过程。
