Espinosa N, Hernández R, López-Griego L, Arroyo R, López-Villaseñor I
Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado postal 70-228, CP 04510, México D.F., Mexico.
Acta Trop. 2001 Feb 23;78(2):147-54. doi: 10.1016/s0001-706x(00)00180-7.
The sequence of a cloned genomic fragment of Trichomonas vaginalis containing a complete actin gene was determined. An uninterrupted open reading frame of 1128 nucleotides was found that codes for an actin gene. Two overlapped consensus promoter sequences for T. vaginalis were found 12 nucleotides upstream the actin initiation codon. In addition to actin, two incomplete open reading frames were found at the 5' and 3' ends of the clone. These two sequences are expressed and showed similarity to adenylate cyclase genes and a yeast hypothetical protein. The overall sequence showed a higher G+C content and a lower frequency of repeated sequences in the coding regions when compared with the non-coding regions. A similar unequal nucleotide distribution was found in various T. vaginalis genes retrieved from data bases.
测定了阴道毛滴虫一个包含完整肌动蛋白基因的克隆基因组片段的序列。发现了一个1128个核苷酸的不间断开放阅读框，其编码一个肌动蛋白基因。在肌动蛋白起始密码子上游12个核苷酸处发现了两个重叠的阴道毛滴虫共有启动子序列。除了肌动蛋白外，在克隆的5'和3'端还发现了两个不完整的开放阅读框。这两个序列可表达，并且与腺苷酸环化酶基因和一种酵母假定蛋白具有相似性。与非编码区相比，整个序列在编码区显示出更高的G+C含量和更低的重复序列频率。在从数据库检索到的各种阴道毛滴虫基因中也发现了类似的核苷酸分布不均现象。
