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[小麦T型细胞质雄性不育系及其保持系线粒体DNA的比较研究]

[Comparative studies of mitochondrial DNA of T-type cytoplasmic male sterile lines and their maintainers in wheat].

作者信息

Qiu Y G, Shen Y Z, Huang Z J, Zhao B C, He C F, Bi C L

机构信息

Hebei Normal University, Life and Science Department, Shijiazhuang 050016, China.

出版信息

Yi Chuan Xue Bao. 2001;28(2):166-70.

PMID:11233261
Abstract

mtDNAs of T type wheat cytoplasmic male sterile lines 75-3369A and their maintainers 75-3369B were isolated and digested completely with restriction endonuclease BamH I, EcoR I, Hind III. The mitochondrial genomic difference between CMS line and maintainers were revealed by Southern hybridization with probes of atp6 from Oenothera and nad3/rps12, cox I from wheat mitochondria. Southern hybridization showed the differences between these two lines. Discussion was made on the change of the DNA composition and the male sterile cytoplasm.

摘要

分离了T型小麦细胞质雄性不育系75 - 3369A及其保持系75 - 3369B的线粒体DNA（mtDNA），并用限制性内切酶BamH I、EcoR I、Hind III进行完全酶切。用月见草atp6以及小麦线粒体nad3/rps12、cox I的探针进行Southern杂交，揭示了不育系和保持系之间线粒体基因组的差异。Southern杂交显示了这两个系之间的差异。对DNA组成的变化和雄性不育细胞质进行了讨论。

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