Rodrigues M, Chehne F, Sinzinger H
Department of Nuclear Medicine, University Hospital, Vienna, Austria.
Nucl Med Commun. 2001 Jan;22(1):105-10. doi: 10.1097/00006231-200101000-00015.
Adsorption of radiopharmaceuticals onto disposable syringes has been reported to amount to levels of almost 50%. Data on adsorption of radiopharmaceuticals onto materials used for in vitro studies are extremely limited. We assessed the extent of adsorption of 99Tc(m) hexakis(2-methoxyisobutylisonitrile) (99Tc(m)-MIBI), 99Tc(m)-tetrofosmin, 99Tc(m)-furifosmin and 99Tc(m)O4 onto tubes used for in vitro measurement of cellular uptake of these radiopharmaceuticals. The influence on adsorption of different incubation media, temperature and time of incubation was evaluated. Total (not corrected for adsorption) uptake was compared with corrected, net cellular uptake in SK-BR-3, MCF-7 and liposarcoma cell lines. Values of adsorption ranging from 0.94+/-0.13% to 7.07+/-0.46% were found. The extent of adsorption of all the radiopharmaceuticals varied with the type of incubation medium and the incubation temperature. With 99Tc(m)-furifosmin, adsorption was dependent on the incubation time as well on the incubation temperature and some of the incubation media investigated. Our findings indicate that systematic investigations to evaluate the adsorption of radiopharmaceuticals onto materials used during in vitro studies of cellular uptake should be considered a mandatory aspect of quality control.
据报道，放射性药物在一次性注射器上的吸附量高达近50%。关于放射性药物在用于体外研究的材料上的吸附数据极为有限。我们评估了99锝（m）六甲基异丁基异腈（99Tc（m）-MIBI）、99Tc（m）-替曲膦、99Tc（m）-呋膦和99Tc（m）O4在用于体外测量这些放射性药物细胞摄取的试管上的吸附程度。评估了不同孵育介质、温度和孵育时间对吸附的影响。将总摄取量（未校正吸附）与SK-BR-3、MCF-7和脂肪肉瘤细胞系中校正后的净细胞摄取量进行比较。发现吸附值范围为0.94±0.13%至7.07±0.46%。所有放射性药物的吸附程度随孵育介质类型和孵育温度而变化。对于99Tc（m）-呋膦，吸附不仅取决于孵育时间，还取决于孵育温度以及一些所研究的孵育介质。我们的研究结果表明，在细胞摄取体外研究中，评估放射性药物在所用材料上的吸附的系统性研究应被视为质量控制的一个必要方面。
