Andersen S M, Jørgensen C, Jacobsen C S
Department of Geochemistry, Geological Survey of Denmark and Greenland, Copenhagen.
Appl Microbiol Biotechnol. 2001 Jan;55(1):112-6. doi: 10.1007/s002530000494.
In this study, we isolated phenanthrene degraders belonging to Pseudomonas spp. by combining the selective force of two previously described media. The two compounds, sodium lauryl sarcosine and trimethoprim, from the Gould S1 medium, were added to minimal agar plates sprayed with phenanthrene. Pseudomonas spp. that could produce clearing zones were isolated in one step from the rhizosphere without first selecting for Pseudomonas spp. and subsequently screening for degraders or vice versa. Enumeration and isolation of Pseudomonas spp. attached to the rhizosphere showed clear differences between two types of soil. Rhizosphere-attached phenanthrene degraders (from Pseudomonas spp.) were isolated from a former coal gasification site, but were absent in an agricultural soil subjected to organic farming. We isolated 23 phenanthrene degraders producing clearing zones from the rhizosphere of barley roots. All of these 23 isolates (of which 16 were fluorescent in UV light) proved to be members of the Pseudomonas RNA homology group I, on the basis of results of the analytical profile index (API) test system and classic taxonomic tests.
在本研究中，我们通过结合两种先前描述的培养基的选择力，分离出了属于假单胞菌属的菲降解菌。将古尔德S1培养基中的两种化合物——月桂酰肌氨酸钠和甲氧苄啶添加到喷洒了菲的基本琼脂平板上。无需先筛选假单胞菌属，再筛选降解菌，反之亦然，一步即可从根际中分离出能产生透明圈的假单胞菌属。对附着在根际的假单胞菌属进行计数和分离，结果显示两种土壤类型之间存在明显差异。从一个 former coal gasification site（原文此处表述不太准确，推测可能是“ former coal gasification plant”，即 former coal gasification site可能是指“ former coal gasification plant”，即前煤气化厂）的根际中分离出了附着根际的菲降解菌（来自假单胞菌属），但在进行有机耕作的农业土壤中则不存在。我们从大麦根际中分离出了23株能产生透明圈的菲降解菌。根据分析谱指数（API）测试系统和经典分类测试的结果，这23株分离菌（其中16株在紫外光下呈荧光）均被证明是假单胞菌RNA同源组I的成员。
