Sun Kai, Liu Juan, Gao Yanzheng, Jin Li, Gu Yujun, Wang Wanqing
1] Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China [2].
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Sci Rep. 2014 Jun 26;4:5462. doi: 10.1038/srep05462.
This investigation provides a novel method of endophyte-aided removal of polycyclic aromatic hydrocarbons (PAHs) from plant bodies. A phenanthrene-degrading endophytic bacterium Pseudomonas sp. Ph6 was isolated from clover (Trifolium pratense L.) grown in a PAH-contaminated site. After being marked with the GFP gene, the colonization and distribution of strain Ph6-gfp was directly visualized in plant roots, stems, and leaves for the first time. After ryegrass (Lolium multiflorum Lam.) roots inoculation, strain Ph6-gfp actively and internally colonized plant roots and transferred vertically to the shoots. Ph6-gfp had a natural capacity to cope with phenanthrene in vitro and in planta. Ph6-gfp degraded 81.1% of phenanthrene (50 mg · L(-1)) in a culture solution within 15 days. The inoculation of plants with Ph6-gfp reduced the risks associated with plant phenanthrene contamination based on observations of decreased concentration, accumulation, and translocation factors of phenanthrene in ryegrass. Our results will have important ramifications in the assessment of the environmental risks of PAHs and in finding ways to circumvent plant PAH contamination.
本研究提供了一种利用内生菌辅助从植物体内去除多环芳烃（PAHs）的新方法。从生长在PAH污染场地的三叶草（Trifolium pratense L.）中分离出一株菲降解内生细菌假单胞菌属Ph6。在用绿色荧光蛋白（GFP）基因标记后，首次直接观察到菌株Ph6 - gfp在植物根、茎和叶中的定殖和分布。黑麦草（Lolium multiflorum Lam.）根系接种后，菌株Ph6 - gfp在植物根系中积极定殖并向地上部分垂直转移。Ph6 - gfp在体外和植物体内均具有处理菲的天然能力。在15天内，Ph6 - gfp在培养液中降解了81.1%的菲（50 mg·L(-1)）。基于对黑麦草中菲的浓度、积累和转运因子降低的观察，用Ph6 - gfp接种植物降低了与植物菲污染相关的风险。我们的研究结果将对评估PAHs的环境风险以及寻找规避植物PAH污染的方法产生重要影响。
