Yoshino M, Kurosaka D, Obazawa M, Takayama F
Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinnjuku-ku, Tokyo 160-8582, Japan.
Nippon Ganka Gakkai Zasshi. 2001 Feb;105(2):83-7.
We investigated whether alpha 5 beta 1 integrin and fibronectin were present in myofibroblast-like lens epithelial cells in anterior subcapsular cataract (ASC).
Nine anterior capsule specimens were obtained from the patients during cataract surgery and frozen for cryostat sections. Six specimens were anterior capsule obtained from cataract with ASC. As a control, three specimens were obtained from cataract without ASC. Alpha-smooth muscle actin (alpha-SMA), alpha 5 beta 1 integrin, and fibronectin were detected by immunohistochemical observation.
In all 6 specimens from patients with ASC, the lens epithelial cells around fibrosis tissue included myofibroblast-like lens epithelial cells which were positive for alpha-SMA. alpha 5 beta 1 integrin was detected in these lens epithelial cells. Fibronectin was also detected around these myofibroblast-like lens epithelial cells. Three control specimens showed no immunoreactivity against alpha-SMA, alpha 5 beta 1 integrin, or fibronectin.
Alpha 5 beta 1 integrin and fibronectin may play an important role in myodifferentiation of lens epithelial cells.
我们研究了α5β1整合素和纤连蛋白是否存在于前囊下白内障（ASC）的肌成纤维细胞样晶状体上皮细胞中。
在白内障手术期间从患者获取9个前囊标本并冷冻以制作冰冻切片。6个标本是从患有ASC的白内障患者获得的前囊。作为对照，3个标本是从无ASC的白内障患者获得的。通过免疫组织化学观察检测α平滑肌肌动蛋白（α-SMA）、α5β1整合素和纤连蛋白。
在所有6个来自ASC患者的标本中，纤维化组织周围的晶状体上皮细胞包括对α-SMA呈阳性的肌成纤维细胞样晶状体上皮细胞。在这些晶状体上皮细胞中检测到α5β1整合素。在这些肌成纤维细胞样晶状体上皮细胞周围也检测到纤连蛋白。3个对照标本对α-SMA、α5β1整合素或纤连蛋白无免疫反应性。
α5β1整合素和纤连蛋白可能在晶状体上皮细胞的肌分化中起重要作用。
