Tsuda T, Horio F, Osawa T
Tokaigakuen Women's College, Nagoya, Japan.
Biofactors. 2000;13(1-4):133-9. doi: 10.1002/biof.5520130122.
Cyanidin 3-O-beta-D-glucoside (C3G) is included in anthocyanins, and expected to have a potency to scavenge active oxygen species in vivo. Rats were fed a diet containing C3G (2 g/kg diet) for 14 days, and then subjected to hepatic ischemia-reperfusion (I/R) as an oxidative stress model. I/R treatment elevated the liver thiobarbituric acid-reactive substance concentration and the serum activities of marker enzymes for liver injury, and lowered the liver reduced glutathione concentration. Feeding C3G significantly suppressed these changes caused by hepatic I/R. These results indicate that C3G functions as a potent antioxidant in vivo under oxidative stress. To clarify the mechanism of action of C3G, we investigated the absorption and metabolism of C3G in rats. C3G appeared in the plasma immediately after the oral administration of C3G. Protocatechuic acid, which seems to be produced by the degradation of cyanidin, was also present in the plasma. In the liver and kidneys, C3G was metabolized to methylated form.
矢车菊素 3 - O - β - D - 葡萄糖苷（C3G）属于花青素，有望在体内具有清除活性氧的能力。给大鼠喂食含C3G（2克/千克饲料）的饲料14天，然后作为氧化应激模型进行肝脏缺血再灌注（I/R）。I/R处理提高了肝脏硫代巴比妥酸反应性物质浓度以及肝脏损伤标志物酶的血清活性，并降低了肝脏还原型谷胱甘肽浓度。喂食C3G可显著抑制肝脏I/R引起的这些变化。这些结果表明，C3G在氧化应激下在体内发挥强效抗氧化剂的作用。为阐明C3G的作用机制，我们研究了C3G在大鼠体内的吸收和代谢情况。口服C3G后，C3G立即出现在血浆中。血浆中还存在似乎由矢车菊素降解产生的原儿茶酸。在肝脏和肾脏中，C3G被代谢为甲基化形式。
