Harp J B, Franklin D, Vanderpuije A A, Gimble J M
Department of Nutrition, University of North Carolina at Chapel Hill, North Carolina 27599, USA.
Biochem Biophys Res Commun. 2001 Mar 9;281(4):907-12. doi: 10.1006/bbrc.2001.4460.
Signal Transducers and Activators of Transcription (STATs) display unique expression patterns upon induction of differentiation of murine 3T3-L1 preadipocytes into adipocytes. During differentiation, expression of STAT1 and STAT5 increase, while STAT3 and STAT6 remain relatively unchanged. Here, we determined whether human subcutaneous preadipocytes expressed STATs and if the pattern of expression changed during adipogenesis. We found by Western blot analysis that freshly isolated preadipocytes expressed STAT1, STAT3, STAT5, and STAT6, but not STAT2 and STAT4. Induction of preadipocyte differentiation with 1-methyl-3-isobutylxanthine, dexamethasone, insulin, and BRL49653 decreased expression of STAT1, and increased expression of STAT3 and STAT5. STAT6 expression did not change during adipogenesis. Changes in expression of CCAAT/enhancer binding protein beta (C/EBPbeta), C/EBPdelta, C/EBPalpha, and peroxisome proliferator-activated receptor gamma were similar to murine cell lines. These results suggest that unlike the traditional adipogenic transcription factors, unique differences exist in STAT expression patterns between murine and human adipose cells.
信号转导子和转录激活子（STATs）在小鼠3T3-L1前脂肪细胞诱导分化为脂肪细胞的过程中呈现出独特的表达模式。在分化过程中，STAT1和STAT5的表达增加，而STAT3和STAT6保持相对不变。在此，我们确定了人皮下前脂肪细胞是否表达STATs，以及在脂肪生成过程中表达模式是否发生变化。我们通过蛋白质印迹分析发现，新鲜分离的前脂肪细胞表达STAT1、STAT3、STAT5和STAT6，但不表达STAT2和STAT4。用1-甲基-3-异丁基黄嘌呤、地塞米松、胰岛素和BRL49653诱导前脂肪细胞分化会降低STAT1的表达，并增加STAT3和STAT5的表达。在脂肪生成过程中，STAT6的表达没有变化。CCAAT/增强子结合蛋白β（C/EBPβ）、C/EBPδ、C/EBPα和过氧化物酶体增殖物激活受体γ的表达变化与小鼠细胞系相似。这些结果表明，与传统的脂肪生成转录因子不同，小鼠和人脂肪细胞之间的STAT表达模式存在独特差异。
