White H D, Musey L K, Andrews M M, Yeaman G R, DeMars L R, Manganiello P D, Howell A L, Wira C R, Green W R, McElrath M J
Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA.
J Infect Dis. 2001 Mar 15;183(6):977-83. doi: 10.1086/319253. Epub 2001 Feb 21.
CD8(+) T cell phenotype and function were assessed in the female reproductive tracts (FRTs) of 3 human immunodeficiency virus (HIV)-positive patients who had undergone hysterectomy. FRT cytotoxic T lymphocyte (CTL) lytic activity from 1 patient (patient 872) was detected by using CD3-dependent redirected-lysis assay and HIV-specific assay, concomitant with the presence of CD8(+) cells. In contrast, samples from the 2 other HIV-positive patients (patients 1356 and 1364), who also were asymptomatic for HIV-associated illnesses, demonstrated no CTL activity in any solid tissue tested by either assay, despite activity by autologous peripheral blood mononuclear cells (PBMC). This absence of CTL activity was correlated with a relative absence of CD8(+) cells in the FRT, whereas CD8(+) cells were present in PBMC. Thus, CTL activity in PBMC may fail to correlate with mucosal activity. The finding of CTL activity in the FRT of patient 872 represents the first description of CTL in upper and lower FRT tissues of an HIV-positive woman.
对3例接受子宫切除术的人类免疫缺陷病毒（HIV）阳性患者的女性生殖道（FRT）中的CD8(+) T细胞表型和功能进行了评估。通过使用CD3依赖性重定向裂解试验和HIV特异性试验，检测到1例患者（患者872）的FRT细胞毒性T淋巴细胞（CTL）裂解活性，同时存在CD8(+) 细胞。相比之下，另外2例HIV阳性患者（患者1356和1364），他们也没有与HIV相关疾病的症状，尽管自体外周血单个核细胞（PBMC）有活性，但通过任何一种试验在测试的任何实体组织中均未显示CTL活性。这种CTL活性的缺乏与FRT中CD8(+) 细胞的相对缺乏相关，而CD8(+) 细胞存在于PBMC中。因此，PBMC中的CTL活性可能与黏膜活性不相关。在患者872的FRT中发现CTL活性代表了对HIV阳性女性上、下FRT组织中CTL的首次描述。
