中央无形体16S rRNA基因序列分析及其与其他埃立克体的系统发育相关性。
Analysis of the 16S rRNA gene sequence of Anaplasma centrale and its phylogenetic relatedness to other ehrlichiae.
作者信息
Inokuma H, Terada Y, Kamio T, Raoult D, Brouqui P
机构信息
Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
出版信息
Clin Diagn Lab Immunol. 2001 Mar;8(2):241-4. doi: 10.1128/CDLI.8.2.241-244.2001.
Abstract
The nucleotide sequence of the Anaplasma centrale 16S rRNA gene was determined and compared with the sequences of ehrlichial bacteria. The sequence of A. centrale was closely related to Anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. A species-specific PCR was developed based upon the alignment data. The PCR can detect A. centrale DNA extracted from 10 infected bovine red blood cells in a reaction mixture. A. centrale DNA was amplified in the reaction, but not other related ehrlichial species.
摘要
测定了中央无形体16S rRNA基因的核苷酸序列，并与埃立克体细菌的序列进行了比较。通过相似性水平（98.08%相同）和距离分析，中央无形体的序列与边缘无形体密切相关。基于比对数据开发了一种种特异性PCR。该PCR可在反应混合物中检测从10个受感染牛红细胞中提取的中央无形体DNA。反应中扩增出了中央无形体DNA，但未扩增出其他相关埃立克体物种的DNA。
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