Laboratoire de Parasitologie, École Nationale de Médecine Vétérinaire de Sidi Thabet, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, Univ. Manouba, Sidi Thabet, Tunisia.
Laboratoire de Parasitologie, Institut des Sciences Vétérinaire El Khroub, Université Frères Mentouri, Constantine 1, Algeria.
Transbound Emerg Dis. 2018 Apr;65(2):456-464. doi: 10.1111/tbed.12725. Epub 2017 Oct 16.
Bovine anaplasmosis could be caused by several Anaplasma species. The causative agents are transmitted by ticks and haematophagous arthropods with a high impact on both human and animal health. This study was conducted to estimate the infection rate and to characterize Anaplasma spp. in cattle from Algeria. A molecular survey was performed in Setif district (Northeast Algeria) where a total number of 180 cattle blood samples were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. PCRs revealed that the infection rates of Anaplasma spp., Anaplasma centrale, Anaplasma marginale and Anaplasma bovis were 42.2%; 39.4%; 11.1% and 4.4%, respectively. All tested animals were negative for A. phagocytophilum. Co-infection occurred in 10% (18/180) of the tested animals, and the most common co-infection pattern was an association between A. centrale and A. marginale (5.5%). Five cattle (2.7%) were co-infected by the three Anaplasma species. Holstein animals (58.1%) were more infected by A. centrale than the other breeds (p = .01). The molecular prevalence of A. centrale was significantly higher in males (54.2%) than in females (34.1%) (p = .001). A. marginale msp4 genetic analysis indicated a high sequence diversity of Algerian strains, suggesting the importation of live cattle from different origins. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. centrale revealed a low degree of genetic diversity. Our study suggests that different species of Anaplasma are simultaneously present in the Algerian cattle. To the best of our knowledge, this is the first molecular study and genetic characterization of Anaplasma spp. in Algerian cattle.
牛无浆体病可由几种无浆体属引起。病原体通过蜱和吸血节肢动物传播，对人类和动物健康都有重大影响。本研究旨在评估阿尔及利亚牛的感染率并对无浆体属进行特征描述。在塞提夫地区（阿尔及利亚东北部）进行了一项分子调查，共采集了 180 份牛血样进行无浆体属的 PCR 检测。根据 16S rRNA 和 msp4 基因对阳性样本进行了遗传特征分析。PCR 显示，无浆体属、中央无浆体、边缘无浆体和牛无浆体的感染率分别为 42.2%、39.4%、11.1%和 4.4%。所有检测的动物均为阴性。10%（18/180）的检测动物发生了混合感染，最常见的混合感染模式是中央无浆体和边缘无浆体的联合感染（5.5%）。有 5 头牛（2.7%）同时感染了三种无浆体属。荷斯坦牛（58.1%）比其他品种（p=.01）更易感染中央无浆体。雄性（54.2%）中央无浆体的分子流行率明显高于雌性（34.1%）（p=.001）。边缘无浆体 msp4 基因遗传分析表明，阿尔及利亚株具有很高的序列多样性，提示从不同来源进口了活牛。牛无浆体和中央无浆体的 16S rRNA 基因系统发育分析显示遗传多样性程度较低。本研究表明，不同种属的无浆体在阿尔及利亚牛中同时存在。据我们所知，这是首次对阿尔及利亚牛无浆体属进行的分子研究和遗传特征描述。
