利用二维聚丙烯酰胺凝胶电泳对边缘无形体和中央无形体进行蛋白质分析。
Protein analysis of Anaplasma marginale and Anaplasma centrale by two-dimensional polyacrylamide gel electrophoresis.
作者信息
Zakimi S, Tsuji N, Fujisaki K
机构信息
Okinawa Prefectural Institute of Animal Health, Japan.
出版信息
J Vet Med Sci. 1994 Oct;56(5):1025-7. doi: 10.1292/jvms.56.1025.
Protein compositions of Anaplasma marginale and A. centrale were analyzed by two-dimensional gel electrophoresis. Both species had a major protein which was composed of 3-4 spots. The molecular weights of these two proteins were approximately 39 kDa. However, the position of these proteins in the gels were slightly different when 2 gel maps were superimposed. Five other protein spots were shared by A. marginale and A. centrale, whereas all the other protein spots were appeared to be unique to each of the species.
采用双向凝胶电泳分析了边缘无形体和中央无形体的蛋白质组成。这两个物种都有一种主要蛋白质，由3 - 4个斑点组成。这两种蛋白质的分子量约为39 kDa。然而，当将两张凝胶图谱叠加时，这些蛋白质在凝胶中的位置略有不同。边缘无形体和中央无形体共有另外5个蛋白质斑点，而所有其他蛋白质斑点似乎是每个物种所特有的。
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