使用Hoechst 33342染色检测感染副结核分枝杆菌亚种的牛单核吞噬细胞中的凋亡变化。
Use of Hoechst 33342 staining to detect apoptotic changes in bovine mononuclear phagocytes infected with Mycobacterium avium subsp. paratuberculosis.
作者信息
Allen S, Sotos J, Sylte M J, Czuprynski C J
机构信息
Department of Bacteriology, College of Agriculture and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
Clin Diagn Lab Immunol. 2001 Mar;8(2):460-4. doi: 10.1128/CDLI.8.2.460-464.2001.
Abstract
Mycobacterium avium subsp. paratuberculosis is an intracellular pathogen of macrophages that causes a chronic enteritis (Johne's disease) in ruminants. The purpose of this study was to determine whether M. avium subsp. paratuberculosis infection causes apoptosis in bovine monocytes. Using Hoechst 33342 staining, we observed increased numbers of apoptotic monocytes within 6 h of infection with M. avium subsp. paratuberculosis, and these numbers increased further at 24 and 48 h. This effect appeared to require viable bacilli, because monocytes infected with heat-killed M. avium subsp. paratuberculosis did not exhibit a significant increase in apoptosis. Preincubation of monocytes with bovine growth hormone prior to infection with M. avium subsp. paratuberculosis did not significantly alter the number of apoptotic cells.
摘要
副结核分枝杆菌是巨噬细胞的一种细胞内病原体，可导致反刍动物患慢性肠炎（副结核病）。本研究的目的是确定副结核分枝杆菌感染是否会导致牛单核细胞凋亡。使用Hoechst 33342染色，我们观察到在感染副结核分枝杆菌后6小时内凋亡单核细胞数量增加，并且在24小时和48小时时这些数量进一步增加。这种效应似乎需要活菌，因为用热灭活的副结核分枝杆菌感染的单核细胞凋亡没有显著增加。在用副结核分枝杆菌感染之前，将单核细胞与牛生长激素预孵育并没有显著改变凋亡细胞的数量。
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本文引用的文献
1
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J Immunol. 2000 Feb 15;164(4):2016-20. doi: 10.4049/jimmunol.164.4.2016.
2
Macrophage control of mycobacterial growth induced by picolinic acid is dependent on host cell apoptosis.巨噬细胞对吡啶甲酸诱导的分枝杆菌生长的控制取决于宿主细胞凋亡。
J Immunol. 2000 Jan 1;164(1):389-97. doi: 10.4049/jimmunol.164.1.389.
3
Hormonal modulation of phagocytosis and intracellular growth of Mycobacterium avium ss. paratuberculosis In bovine peripheral blood monocytes.禽分枝杆菌副结核亚种在牛外周血单核细胞中吞噬作用和细胞内生长的激素调节
Microb Pathog. 1999 Jan;26(1):1-11. doi: 10.1006/mpat.1998.0246.
4
Interferon-gamma and interleukin 4 gene expression in cows infected with Mycobacterium paratuberculosis.感染副结核分枝杆菌的奶牛中γ干扰素和白细胞介素4基因的表达
Am J Vet Res. 1998 Jul;59(7):842-7.
5
Thermal tolerance of Mycobacterium paratuberculosis.副结核分枝杆菌的热耐受性
Appl Environ Microbiol. 1998 Mar;64(3):999-1005. doi: 10.1128/AEM.64.3.999-1005.1998.
6
Apoptosis of human monocytes and macrophages by Mycobacterium avium sonicate.鸟分枝杆菌超声裂解物诱导人单核细胞和巨噬细胞凋亡
Infect Immun. 1997 Dec;65(12):5262-71. doi: 10.1128/iai.65.12.5262-5271.1997.
7
Effects of gamma interferon and nitric oxide on the interaction of Mycobacterium avium subsp. paratuberculosis with bovine monocytes.γ干扰素和一氧化氮对副结核分枝杆菌与牛单核细胞相互作用的影响。
Infect Immun. 1997 May;65(5):1761-6. doi: 10.1128/iai.65.5.1761-1766.1997.
8
Apoptosis of human monocytes/macrophages in Mycobacterium tuberculosis infection.结核分枝杆菌感染中人类单核细胞/巨噬细胞的凋亡
J Pathol. 1997 Jan;181(1):31-8. doi: 10.1002/(SICI)1096-9896(199701)181:1<31::AID-PATH722>3.0.CO;2-G.
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