Olatunbosun O, Deneer H, Pierson R
Department of Obstetrics, Gynecology and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Obstet Gynecol. 2001 Mar;97(3):357-60. doi: 10.1016/s0029-7844(00)01183-2.
To detect human papillomavirus (HPV) in semen and find if sperm washing removes HPV DNA.
Amplification by nested polymerase chain reaction (PCR) was used to detect viral DNA sequences in semen samples from 85 volunteers. Forty-five men had historical or clinical evidence of genital HPV infection (study group) and 40 were healthy, clinically HPV-negative semen donors.
We detected HPV DNA in the sperm cells of 24 of 45 subjects (53%) with past or current HPV infections in contrast to three of 40 healthy subjects (8%) (P <.001). Overall, PCR detected HPV in 21 of 32 subjects (66%) with identifiable lesions and six of 53 (11%) without them (P <.001). Swim-up washings of all 27 prewash sperm cells with HPV reduced cellular HPV DNA below detectable levels in only two cases.
HPV is present in sperm cells from infected and apparently healthy subjects, and sperm washing does not eliminate the risk of HPV transmission to recipients. We suggest that HPV DNA testing should be done on the semen of prospective donors, and those with positive tests should be excluded from donation.
检测精液中的人乳头瘤病毒（HPV），并确定精子洗涤是否能去除HPV DNA。
采用巢式聚合酶链反应（PCR）扩增法检测85名志愿者精液样本中的病毒DNA序列。45名男性有生殖器HPV感染的既往史或临床证据（研究组），40名是健康的、临床HPV阴性的精液捐献者。
我们在45名既往或当前感染HPV的受试者中的24名（53%）精子细胞中检测到了HPV DNA，相比之下，40名健康受试者中有3名（8%）检测到（P<.001）。总体而言，PCR在32名有可识别病变的受试者中的21名（66%）检测到HPV，在53名无病变的受试者中的6名（11%）检测到（P<.001）。对所有27个洗涤前含有HPV的精子细胞进行上浮洗涤，仅在2例中使细胞HPV DNA降至检测水平以下。
HPV存在于感染和表面健康受试者的精子细胞中，精子洗涤不能消除HPV传播给受者的风险。我们建议应对潜在捐献者的精液进行HPV DNA检测，检测呈阳性者应被排除在捐献之外。
