Lai Y M, Lee J F, Huang H Y, Soong Y K, Yang F P, Pao C C
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan, Republic of China.
Fertil Steril. 1997 Jun;67(6):1152-5. doi: 10.1016/s0015-0282(97)81454-9.
To investigate the presence of human papillomavirus (HPV) in human sperm cells and to evaluate potential effects of HPV on the sperm functions.
A descriptive clinical study.
PATIENT(S): Specimens of semen were collected from 24 randomly selected patients who attended the fertility clinics at Chang Gung Memorial Hospital.
MAIN OUTCOME MEASURE(S): The presence of HPV DNA and RNA were examined by polymerase chain reaction. Semen quality and sperm cell function were analyzed by computer-aided autoanalyzer.
RESULT(S): Human papillomavirus type 16 DNA and RNA were found in 6 (25%) and 2 (8%) of the sperm cells specimens, respectively. Human papillomavirus type 18 DNA and RNA were present in 11 (46%) and 5 (21%) of the same sperm cells specimens, respectively. Incidence of asthenozoospermia among patients infected with either HPV was significantly higher than in those without HPV in their sperm cells (75% versus 8%). Although performance of curvilinear velocity, straight-line velocity, and mean amplitude of lateral head displacement was significantly lower in HPV-infected specimens, the differences of linearity, beat cross frequency, and straightness were not statistically significant.
CONCLUSION(S): These results suggest that human papillomavirus can be found in human sperm cells and that certain HPV-specific genes are actively transcribed. Sperm motility parameters seem to be affected by the presence of HPV in the sperm cells, and also the incidence of asthenozoospermia may be associated with HPV infection.
研究人类精子细胞中人类乳头瘤病毒（HPV）的存在情况，并评估HPV对精子功能的潜在影响。
一项描述性临床研究。
从长庚纪念医院生殖科随机选取24名患者采集精液样本。
采用聚合酶链反应检测HPV DNA和RNA的存在情况。通过计算机辅助自动分析仪分析精液质量和精子细胞功能。
在精子细胞样本中，分别有6份（25%）和2份（8%）检测到16型HPV DNA和RNA。在相同的精子细胞样本中，分别有11份（46%）和5份（21%）检测到18型HPV DNA和RNA。精子细胞感染HPV的患者中弱精子症的发生率显著高于未感染HPV的患者（75%对8%）。虽然HPV感染样本的曲线速度、直线速度和平均侧摆幅值显著降低，但线性度、鞭打交叉频率和直线性的差异无统计学意义。
这些结果表明，人类精子细胞中可发现HPV，且某些HPV特异性基因处于活跃转录状态。精子细胞中HPV的存在似乎会影响精子活力参数，弱精子症的发生率也可能与HPV感染有关。
