Ochs H, Lonneux J, Losson B J, Deplazes P
Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, CH-8057, Zurich, Switzerland.
Vet Parasitol. 2001 Apr 2;96(3):233-42. doi: 10.1016/s0304-4017(00)00444-1.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of specific antibodies against crude Psoroptes antigen. The diagnostic sensitivity was 93.7% in 191 sheep with clinical signs associated with mange. These animals originated from 29 flocks in which psoroptic mites were detected. All of 59 sheep infested with Psoroptes ovis were seropositive. Additionally, in 49% of 70 clinically unaffected sheep originating from P. ovis-infested flocks, specific antibodies could be detected, suggesting that asymptomatic infestations can be diagnosed by serology. The specificity of the ELISA was 96.5% as determined with 254 sheep originating from 44 flocks without clinical mange. Cross-reactivity in a low range was detected with selected sera of sheep with clinical chorioptic or forage mite infestations. Four sheep seroconverted 2 weeks after experimental P. ovis infestation, i.e. 2 weeks before clinical signs became obvious. After successful doramectin treatment of 14 sheep with naturally acquired P. ovis infestation, the ELISA values declined slowly but remained positive in seven cases beyond 17 weeks.
开发了一种酶联免疫吸附测定法（ELISA）用于检测针对粗制痒螨抗原的特异性抗体。在191只出现与疥癣相关临床症状的绵羊中，诊断敏感性为93.7%。这些动物来自29个检测到痒螨的羊群。所有59只感染绵羊痒螨的绵羊血清均呈阳性。此外，在来自感染绵羊痒螨羊群的70只临床上未受影响的绵羊中，49%能够检测到特异性抗体，这表明无症状感染可以通过血清学诊断。用来自44个无临床疥癣羊群的254只绵羊测定，ELISA的特异性为96.5%。在患有临床绒毛螨或草料螨感染的绵羊的选定血清中检测到低水平的交叉反应。4只绵羊在实验性感染绵羊痒螨2周后血清转化，即在临床症状明显出现前2周。在用多拉菌素成功治疗14只自然感染绵羊痒螨的绵羊后，ELISA值缓慢下降，但7只绵羊在17周后仍呈阳性。
