Kubota-Aizawa Sanae, Ohno Koichi, Fukushima Kenjiro, Kanemoto Hideyuki, Nakashima Ko, Uchida Kazuyuki, Chambers James K, Goto-Koshino Yuko, Watanabe Takayasu, Sekizaki Tsutomu, Mimuro Hitomi, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Vet J. 2017 Jul;225:56-62. doi: 10.1016/j.tvjl.2017.04.004. Epub 2017 May 3.
Epidemiological and pathological studies of Helicobacter spp. in canine stomachs in Japan were performed to investigate strain specific pathogenicity. Gastric biopsies from 144 dogs with gastrointestinal diseases were evaluated for the presence of Helicobacter spp. using genus and species specific PCRs for Helicobacter felis, Helicobacter bizzozeronii, Helicobacter heilmannii sensu stricto (s.s.) and Helicobacter pylori. PCR indicated that 50/144 (34.7%) dogs were infected with Helicobacter spp. Of the genus positive samples, 21/50 could not be amplified by any of the species specific PCRs. To investigate Helicobacter at the species level, partial ureAB gene sequences from 48/50 genus positive samples were determined; 47 strains were identified. Thirty-five strains from 45 cases were closely related to H. heilmannii s.s. (89-99% sequence similarity), seven strains from seven cases were closely related to H. bizzozeronii (95-99% sequence similarity), three strains from three cases were closely related to Helicobacter felis (86%, 98% and 99% sequence similarity), one strain from one case was closely related to Helicobacter salomonis (99% sequence similarity) and one strain from one case was closely related to H. pylori (99% sequence similarity). Dogs infected with Helicobacter spp. most similar to H. heilmannii s.s. had a higher frequency of moderate to severe gastritis than dogs negative for Helicobacter spp. (P=0.044). In conclusion, the predominant Helicobacter spp. detected in canine stomachs in our study were most closely related to H. heilmannii s.s. and displayed substantial genetic diversity. Infection with Helicobacter spp. may be associated with more severe gastritis in dogs.
在日本，对犬胃中的螺杆菌属进行了流行病学和病理学研究，以调查菌株特异性致病性。使用针对猫螺杆菌、比氏螺杆菌、狭义海尔曼螺杆菌和幽门螺杆菌的属特异性和种特异性聚合酶链反应（PCR），对144只患有胃肠疾病的犬的胃活检样本进行了螺杆菌属检测。PCR结果显示，144只犬中有50只（34.7%）感染了螺杆菌属。在属阳性样本中，有21/50的样本无法通过任何种特异性PCR进行扩增。为了在种水平上研究螺杆菌，测定了50个属阳性样本中48个样本的部分ureAB基因序列；鉴定出47株菌株。45例中的35株与狭义海尔曼螺杆菌密切相关（序列相似性为89 - 99%），7例中的7株与比氏螺杆菌密切相关（序列相似性为95 - 99%），3例中的3株与猫螺杆菌密切相关（序列相似性分别为86%、98%和99%），1例中的1株与所罗门螺杆菌密切相关（序列相似性为99%），1例中的1株与幽门螺杆菌密切相关（序列相似性为99%）。感染与狭义海尔曼螺杆菌最相似的螺杆菌属的犬，中度至重度胃炎的发生率高于螺杆菌属阴性的犬（P = 0.044）。总之，在我们的研究中，犬胃中检测到的主要螺杆菌属与狭义海尔曼螺杆菌关系最为密切，并表现出显著的遗传多样性。犬感染螺杆菌属可能与更严重的胃炎有关。
