Derst C, Karschin C, Wischmeyer E, Hirsch J R, Preisig-Müller R, Rajan S, Engel H, Grzeschik K, Daut J, Karschin A
Institute for Normal and Pathological Physiology, University of Marburg, Germany.
FEBS Lett. 2001 Mar 2;491(3):305-11. doi: 10.1016/s0014-5793(01)02202-5.
We have identified several cDNAs for the human Kir5.1 subunit of inwardly rectifying K(+) channels. Alternative splicing of exon 3 and the usage of two alternative polyadenylation sites contribute to cDNA diversity. The hKir5.1 gene KCNJ16 is assigned to chromosomal region 17q23.1-24.2, and is separated by only 34 kb from the hKir2.1 gene (KCNJ2). In the brain, Kir5.1 mRNA is restricted to the evolutionary older parts of the hindbrain, midbrain and diencephalon and overlaps with Kir2.1 in the superior/inferior colliculus and the pontine region. In the kidney Kir5.1 and Kir2.1 are colocalized in the proximal tubule. When expressed in Xenopus oocytes, Kir5.1 is efficiently targeted to the cell surface and forms electrically silent channels together with Kir2.1, thus negatively controlling Kir2.1 channel activity in native cells.
我们已经鉴定出内向整流钾离子通道的人类Kir5.1亚基的几个cDNA。外显子3的可变剪接和两个可变聚腺苷酸化位点的使用导致了cDNA的多样性。hKir5.1基因KCNJ16定位于染色体区域17q23.1 - 24.2，与hKir2.1基因（KCNJ2）仅相隔34 kb。在大脑中，Kir5.1 mRNA局限于后脑、中脑和间脑进化较古老的部分，并且在上下丘和脑桥区域与Kir2.1重叠。在肾脏中，Kir5.1和Kir2.1共定位于近端小管。当在非洲爪蟾卵母细胞中表达时，Kir5.1有效地靶向细胞表面，并与Kir2.1一起形成电沉默通道，从而在天然细胞中负向控制Kir2.1通道活性。
