Ye Q, Ohsaki K, Li K, Li D J, Zhu C S, Ogawa T, Tenshin S, Takano-Yamamoto T
Department of Pathology, School of Medicine, University of Tokushima, 770-8503, Tokushima, Japan.
Auris Nasus Larynx. 2001 Apr;28(2):131-6. doi: 10.1016/s0385-8146(00)00079-1.
Present study was performed to evaluate the histological response of rat middle ear mucosa following implantation of Apaceram granules, a synthetic dense hydroxyapatite [Ca10(PO4)6(OH)2], prepared from commercially available synthetic auditory ossicle, and to assess the precise histological response of the rat middle ear to implantation of Apaceram granules, by microscopic examination of mucosal tissue at various time points after implantation.
Apaceram granules were implanted in the temporal bulla of 32 rats. As control, sham surgery was performed in a group of ten rats. Bulla specimens were removed at 1, 3, 7, 14, and 30 days after surgery in the implant and control groups, and at 90, 180 and 300 days in the implant group. Specimens were decalcified, sectioned at a thickness of 6 microm, and stained with haematoxylin and eosin, and Mallory's azan for histological examination of mucosal tissue.
Evidence of inflammatory reaction was slightly greater in the implant group than in controls. Lymphocyte and macrophage counts were higher in the implant group 1 day after surgery, but decreased to similar levels by day 3, and continued to decrease thereafter, and few were observed in the implant group at 300 days. Neutrophils observed at 1 day after surgery were not evident in either group at 3 days. Gradual fibrosis development continued in both groups over all time points studied. Foreign body giant cells were never observed in either group. No bony reaction was observed in any specimen.
The results of this study suggest that Apaceram is biocompatible and suitable for reconstructive ear surgery.
本研究旨在评估Apaceram颗粒植入大鼠中耳黏膜后的组织学反应。Apaceram颗粒是一种合成致密羟基磷灰石[Ca10(PO4)6(OH)2]，由市售人工听小骨制备而成。通过在植入后不同时间点对黏膜组织进行显微镜检查，评估大鼠中耳对Apaceram颗粒植入的精确组织学反应。
将Apaceram颗粒植入32只大鼠的颞骨鼓室。作为对照，对一组10只大鼠进行假手术。在植入组和对照组中，分别于术后1、3、7、14和30天取出鼓室标本，植入组还于术后90、180和300天取出标本。将标本脱钙，切成6微米厚的切片，用苏木精和伊红以及马洛里氏azan染色，用于黏膜组织的组织学检查。
植入组的炎症反应证据略多于对照组。植入组术后1天淋巴细胞和巨噬细胞计数较高，但到第3天降至相似水平，此后继续下降，在植入组300天时很少观察到。术后1天观察到的中性粒细胞在术后3天两组中均未明显见到。在所有研究时间点，两组均持续出现逐渐发展的纤维化。两组均未观察到异物巨细胞。任何标本中均未观察到骨反应。
本研究结果表明，Apaceram具有生物相容性，适用于耳重建手术。
