Cui P C, Ohsaki K, It K, Tenshin S, Kawata T
Division of Clinical Otology, School of Medicine, University of Tokushima, Japan.
J Laryngol Otol. 1995 Jan;109(1):14-8. doi: 10.1017/s0022215100129135.
A study was carried out in order to obtain further information about the soft tissue response to thin Apaceram discs of dense hydroxyapatite (HA) implanted in rats for various periods of time between one day and 10 months. The Apaceram discs were implanted subcutaneously into the interscapular region of 33 rats. A sham operation was performed on eight rats used as controls. Decalcified histological sections stained with haematoxylin and eosin and Mallory's azan were examined and the different cell types found around the implants were counted. It was found that an acute inflammatory reaction occurred after one day and disappeared at about two weeks after implantation. In the test groups, macrophages and lymphocytes disappeared about one week later, and no inflammatory reaction was observed from one to three months. However, a tissue reaction occurred at six months with the appearance of macrophages and lymphocytes, and decreased gradually at 10 months. Meanwhile, a few foreign body giant cells at the Apaceram-tissue interface and a thick layer of fibrous connective tissue around the Apaceram disc were observed at 10 months. No osteogenesis was observed in any specimen. The results obtained so far suggest that Apaceram is still a useful material for reconstructive surgery, despite the possible appearance of a slight macrophage reaction at six months.
为了获取更多关于致密羟基磷灰石（HA）制成的薄Apaceram盘植入大鼠体内1天至10个月不同时间段后软组织反应的信息，开展了一项研究。将Apaceram盘皮下植入33只大鼠的肩胛间区域。对8只用作对照的大鼠进行假手术。检查用苏木精-伊红和马洛里氏azan染色的脱钙组织切片，并对植入物周围发现的不同细胞类型进行计数。结果发现，植入后1天出现急性炎症反应，并在植入后约2周消失。在试验组中，巨噬细胞和淋巴细胞约1周后消失，1至3个月未观察到炎症反应。然而，6个月时出现了组织反应，伴有巨噬细胞和淋巴细胞出现，并在10个月时逐渐减轻。同时，在10个月时观察到Apaceram与组织界面处有一些异物巨细胞，且Apaceram盘周围有一层厚厚的纤维结缔组织。在任何标本中均未观察到骨生成。目前获得的结果表明，尽管6个月时可能会出现轻微的巨噬细胞反应，但Apaceram仍是重建手术的一种有用材料。
