della Torre A, Fanello C, Akogbeto M, Dossou-yovo J, Favia G, Petrarca V, Coluzzi M
Istituto di Parassitologia, Fondazione Pasteur - Cenci Bolognetti, Università di Roma La Sapienza, Piazzale Aldo Moro 5, 00185, Rome, Italy.
Insect Mol Biol. 2001 Feb;10(1):9-18. doi: 10.1046/j.1365-2583.2001.00235.x.
We karyotyped and identified by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis Anopheles gambiae s.s. samples collected in several African countries. The data show the existence of two non-panmictic molecular forms, named S and M, whose distribution extended from forest to savannahs. Mosquitoes of the S and M forms are homosequential standard for chromosome-2 inversions in forest areas. In dry savannahs, S is characterized mainly by inversion polymorphisms typical of Savanna and Bamako chromosomal forms, while M shows chromosome-2 arrangements typical of Mopti and/or Savanna and/or Bissau, depending on its geographical origin. Chromosome-2 inversions therefore seem to be involved in ecotypic adaptation rather than in mate-recognition systems. Strong support for the reproductive isolation of S and M in Ivory Coast comes from the observation that the kdr allele is found at high frequencies in S specimens and not at all in chromosomal identical M specimens. However, the kdr allele does not segregate with molecular forms in Benin.
我们通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析对在几个非洲国家采集的冈比亚按蚊指名亚种样本进行了核型分析和鉴定。数据显示存在两种非随机交配的分子形式，命名为S和M，其分布从森林延伸至稀树草原。S型和M型蚊子在森林地区是2号染色体倒位的同序列标准型。在干旱稀树草原地区，S型主要以稀树草原和巴马科染色体形式典型的倒位多态性为特征，而M型则根据其地理起源显示出莫普提和/或稀树草原和/或比绍典型的2号染色体排列。因此，2号染色体倒位似乎参与了生态型适应而非交配识别系统。在象牙海岸，S型和M型存在生殖隔离的有力证据来自于以下观察结果：kdr等位基因在S型样本中高频出现，而在染色体相同的M型样本中根本不存在。然而，在贝宁，kdr等位基因并不与分子形式发生分离。
