Favia G, Lanfrancotti A, Spanos L, Sidén-Kiamos I, Louis C
Istituto di Parassitologia, Fondazione Cenci-Bolognetti, Università di Roma La Sapienza, Piazzale Aldo Moro 5, 00185, Rome, Italy.
Insect Mol Biol. 2001 Feb;10(1):19-23. doi: 10.1046/j.1365-2583.2001.00236.x.
The sequence of a 2.3 kb long DNA segment derived from the 5'-most end of the ribosomal intergenic spacer was determined in three chromosomal forms of Anopheles gambiae s.s. The analysis revealed that the sequence of the Mopti form differed from that of the Bamako and Savanna forms by a total of ten nucleotide substitutions. Using these sequence variations we set up a diagnostic polymerase chain reaction (PCR) assay to distinguish mosquitoes belonging to the three chromosomal forms, facilitating studies on the distribution and the ecology of these incipient taxa. The assay also allows to distinguish whether a given specimen could represent a heterozygote between Mopti and Savanna or Bamako.
测定了冈比亚按蚊指名亚种三种染色体形式中源自核糖体基因间隔区5'最末端的一段2.3 kb长DNA片段的序列。分析表明，莫普提型的序列与巴马科型和萨凡纳型的序列共有10个核苷酸替换差异。利用这些序列变异，我们建立了一种诊断性聚合酶链反应（PCR）检测方法，以区分属于这三种染色体形式的蚊子，便于对这些初始分类单元的分布和生态进行研究。该检测方法还能够区分给定标本是否可能代表莫普提型与萨凡纳型或巴马科型之间的杂合子。
