Mitsunobu F, Mifune T, Hosaki Y, Ashida K, Tsugeno H, Okamoto M, Takata S, Tanizaki Y
Department of Medicine, Misasa Medical Branch, Okayama University Medical School, Japan.
J Allergy Clin Immunol. 2001 Mar;107(3):492-8. doi: 10.1067/mai.2001.112694.
How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear.
Our goal was to determine whether LTB4 and LTC4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD.
We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB4 and LTC4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens.
The leukocytes of patients with COPD generated significantly more LTB4 (with allergy, P <.001; without allergy, P <.001) and LTC4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB4 generated was not significantly different. FEV(1) significantly correlated with the level of both LTB4 (with allergy, r = -0.556, P =.0375; without allergy, r = -0.731, P =.0046) and LTC4 (with allergy, r = -0.764, P =.0043; without allergy, r = -0.526, P =.0414) generation in COPD. The log(10) of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05).
Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC4 generation, bronchial hyperresponsiveness, and the relationship between LTC4 generation and airway obstruction.
白三烯（LTs）和IgE介导的过敏反应如何反映慢性阻塞性肺疾病（COPD）患者的临床特征仍不清楚。
我们的目标是确定LTB4和LTC4是否与气道阻塞相关，以及IgE介导的过敏反应是否会影响COPD患者LTs的产生和支气管高反应性。
我们测量了肺功能、乙酰甲胆碱支气管高反应性，以及在存在针对吸入性过敏原的特异性IgE抗体的情况下，用钙离子载体A23187刺激外周血白细胞产生LTB4和LTC4的情况。
与对照组相比，COPD患者的白细胞产生的LTB4（有过敏，P<.001；无过敏，P<.001）和LTC4（有过敏，P<.001；无过敏，P<.01）明显更多。过敏的COPD患者的LTC4产生量显著高于非过敏的COPD患者（P<.01），但产生的LTB4量没有显著差异。在COPD患者中，第一秒用力呼气容积（FEV(1)）与LTB4（有过敏，r = -0.556，P =.0375；无过敏，r = -0.731，P =.0046）和LTC4（有过敏，r = -0.764，P =.0043；无过敏，r = -0.526，P =.0414）的产生水平均显著相关。无过敏的COPD患者的乙酰甲胆碱最小剂量的对数显著高于有过敏的患者（P<.05）。
在COPD患者中观察到外周血白细胞产生LTs增加，针对吸入性过敏原的特异性IgE抗体的存在增强了LTC4的产生、支气管高反应性以及LTC4产生与气道阻塞之间的关系。
