Relas H, Gylling H, Miettinen T A
Division of Internal Medicine, Department of Medicine, University of Helsinki, PO Box 340, FIN-00029 HYKS, Helsinki, Finland.
Br J Nutr. 2001 Feb;85(2):141-7. doi: 10.1079/bjn2000231.
Stanyl esters dissolved in margarine inhibit cholesterol absorption, lower sterol absorption in general, and lower serum total cholesterol, LDL-cholesterol and plant sterol levels. To find out whether stanyl esters inhibit absorption of fat-soluble vitamins and beta-carotene in acute experiments, we performed two fat-tolerance tests fortified with vitamins (retinol 0.9-3.7 mg, alpha-tocopherol 70-581 mg), beta-carotene (25-150 mg) and squalene (0.5 g) with and without 1 g of stanyl ester added to the test meal in ten healthy men. The concentrations or areas under the curves (AUC) of cholesterol, triacylglycerols, squalene and alpha-tocopherol, beta-carotene and retinyl palmitate showed typical postprandial changes in serum, chylomicrons, VLDL and VLDL infranatant (intermediate-density lipoproteins, LDL and HDL) over 24 h after the test meal without stanyl esters, and they were not affected by the addition of stanyl esters. The post-absorptive serum campesterol concentration and campesterol : cholesterol were significantly lowered at 6-9 h by stanyl ester supplementation, reflecting reduced sterol absorption efficiency. Changes in vitamin and beta-carotene AUC did not correlate with the given doses. In conclusion, the present study shows that stanyl esters dissolved in margarine do not detectably interfere in a short-term study with the absorption of alpha-tocopherol, beta-carotene or retinol measured by a 24 h oral fat-load test.
溶解在人造黄油中的司他汀酯可抑制胆固醇吸收，总体上降低甾醇吸收，并降低血清总胆固醇、低密度脂蛋白胆固醇和植物甾醇水平。为了在急性实验中探究司他汀酯是否抑制脂溶性维生素和β-胡萝卜素的吸收，我们对10名健康男性进行了两项脂肪耐量试验，试验餐中添加了维生素（视黄醇0.9 - 3.7毫克、α-生育酚70 - 581毫克）、β-胡萝卜素（25 - 150毫克）和角鲨烯（0.5克），其中一项添加了1克司他汀酯，另一项未添加。胆固醇、三酰甘油、角鲨烯以及α-生育酚、β-胡萝卜素和视黄醇棕榈酸酯的浓度或曲线下面积（AUC）显示，在未添加司他汀酯的试验餐后24小时内，血清、乳糜微粒、极低密度脂蛋白和极低密度脂蛋白下层（中密度脂蛋白、低密度脂蛋白和高密度脂蛋白）出现了典型的餐后变化，添加司他汀酯并未对其产生影响。补充司他汀酯后，6 - 9小时的吸收后血清菜油甾醇浓度和菜油甾醇:胆固醇显著降低，反映出甾醇吸收效率降低。维生素和β-胡萝卜素AUC的变化与给定剂量无关。总之，本研究表明，在短期研究中，溶解在人造黄油中的司他汀酯不会对通过24小时口服脂肪负荷试验测量的α-生育酚、β-胡萝卜素或视黄醇的吸收产生明显干扰。
