非裔美国人总体样本中的β3-肾上腺素能受体基因与肥胖
The beta 3-adrenergic receptor gene and obesity in a population sample of African Americans.
作者信息
Lowe W L, Rotimi C N, Luke A, Guo X, Zhu X, Comuzzie A G, Schuh T S, Halbach S, Kotlar T J, Cooper R S
机构信息
Department of Medicine, Northwestern University Medical School and VA Chicago Healthcare System, Lakeside Division, Chicago, Illinois 60611, USA.
出版信息
Int J Obes Relat Metab Disord. 2001 Jan;25(1):54-60. doi: 10.1038/sj.ijo.0801487.
OBJECTIVE
To examine the role of the Trp64Arg polymorphism in the beta 3-adrenergic receptor gene and the beta 3-adrenergic receptor gene locus in obesity-related traits in African Americans.
SUBJECTS
A total of 687 individuals representing 193 African American families who were residents of metropolitan Chicago.
MEASUREMENTS
Genotyping of the Trp64Arg polymorphism in the beta 3-adrenergic receptor gene and three microsatellite markers flanking the beta 3-adrenergic receptor gene (ADRB3) locus and measuring various obesity-related traits, including body mass index (BMI), fat-free mass, fat mass, percentage fat mass, waist circumference and serum lipid levels.
RESULTS
The prevalence of obesity (defined as body mass index > or = 30 kg/m(2)) in the population was 27.3% and 51.2% in men and women, respectively. The frequency of the Arg64 allele was 10.0%. Multivariate regression analyses confirmed the existence of a significant contribution of familial variance to each of the five obesity-related traits noted above. Likelihood ratio statistics computed in a multivariate regression analysis failed to demonstrate a significant association between the Arg64 allele and any of the five obesity-related traits. Single and multipoint analyses using extended Haseman--Elston regression analyses failed to demonstrate suggestive evidence of linkage of three microsatellite markers that flank the beta 3-adrenergic receptor gene to BMI, percentage body fat, waist circumference or serum leptin levels.
CONCLUSION
Given the contribution of familial variance to obesity-related traits in this population, neither the null finding for the Arg64 allele nor the lack of evidence of linkage of the ADRB3 locus to obesity-related traits could be attributed to lack of transmissibility of the traits suggesting that neither the Arg64 variant of the beta 3-adrenergic receptor gene nor another genetic variant in or near the ADRB3 locus contribute significantly to familial aggregation of obesity-related traits in African Americans. International Journal of Obesity (2001) 25, 54-60
目的
研究β3-肾上腺素能受体基因中的色氨酸64精氨酸（Trp64Arg）多态性以及β3-肾上腺素能受体基因位点在非裔美国人肥胖相关性状中的作用。
对象
共687名个体，代表居住在芝加哥大都市的193个非裔美国家庭。
测量指标
对β3-肾上腺素能受体基因中的Trp64Arg多态性以及β3-肾上腺素能受体基因（ADRB3）位点侧翼的三个微卫星标记进行基因分型，并测量各种肥胖相关性状，包括体重指数（BMI）、去脂体重、脂肪量、脂肪量百分比、腰围和血脂水平。
结果
该人群中肥胖（定义为体重指数≥30 kg/m²）的患病率在男性和女性中分别为27.3%和51.2%。精氨酸64等位基因的频率为10.0%。多变量回归分析证实家族变异对上述五种肥胖相关性状均有显著贡献。多变量回归分析中计算的似然比统计未能显示精氨酸64等位基因与上述五种肥胖相关性状中的任何一种之间存在显著关联。使用扩展的哈斯曼-埃尔斯顿回归分析进行的单变量和多变量分析未能显示β3-肾上腺素能受体基因侧翼的三个微卫星标记与BMI、体脂百分比、腰围或血清瘦素水平之间存在连锁的提示性证据。
结论
鉴于家族变异对该人群肥胖相关性状的影响，精氨酸64等位基因的阴性结果以及ADRB3位点与肥胖相关性状缺乏连锁证据均不能归因于性状缺乏可遗传性，这表明β3-肾上腺素能受体基因的精氨酸64变体或ADRB3位点内或附近的另一个基因变体对非裔美国人肥胖相关性状的家族聚集没有显著贡献。《国际肥胖杂志》（2001年）25卷，54 - 60页
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