González-Rodríguez G, Ocádiz-Delgado R
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM y División de Investigación Básica, Instituto Nacional de Cancerología-SS, México.
Gac Med Mex. 2001 Jan-Feb;137(1):15-20.
Actinic prurigo (AP) is a chronic photodermatosis in which genetic and immunological factors has been implicated in the pathogenesis of the disease. This work was designed to investigate the existence of clonal populations of T and B lymphocytes in lesions of the labial mucosa and conjunctiva of patients with actinic prurigo. Genomic DNA of three patients with actinic prurigo and controls were analyzed in Southern blots using DNA molecular probes for the b subunit of the T-cell receptor for antigen (TCR beta) and for the heavy chain of immunoglobulin genes (lg-JH). Clonal rearrangements of T-cell receptor genes were detected in biopsy samples taken from the labial mucosa of two patients and of immunoglobulin genes in DNA extracted from the conjunctiva of a different patient. The presence of distinct clonal T or B lymphocyte populations in patients with actinic prurigo indicates that the immune system may play a relevant role in the pathogenesis of the disease.
光化性痒疹（AP）是一种慢性光皮肤病，其发病机制涉及遗传和免疫因素。本研究旨在调查光化性痒疹患者唇黏膜和结膜病变中T和B淋巴细胞克隆群体的存在情况。使用针对抗原的T细胞受体β亚基（TCRβ）和免疫球蛋白基因重链（Ig-JH）的DNA分子探针，对3例光化性痒疹患者和对照者的基因组DNA进行Southern印迹分析。在取自2例患者唇黏膜的活检样本中检测到T细胞受体基因的克隆重排，在取自另1例患者结膜的DNA中检测到免疫球蛋白基因的克隆重排。光化性痒疹患者中存在不同的克隆性T或B淋巴细胞群体，这表明免疫系统可能在该疾病的发病机制中发挥相关作用。
