Boldogh I R, Yang H C, Nowakowski W D, Karmon S L, Hays L G, Yates J R, Pon L A
Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3162-7. doi: 10.1073/pnas.051494698.
The Arp2/3 complex is implicated in actin polymerization-driven movement of Listeria monocytogenes. Here, we find that Arp2p and Arc15p, two subunits of this complex, show tight, actin-independent association with isolated yeast mitochondria. Arp2p colocalizes with mitochondria. Consistent with this result, we detect Arp2p-dependent formation of actin clouds around mitochondria in intact yeast. Cells bearing mutations in ARP2 or ARC15 genes show decreased velocities of mitochondrial movement, loss of all directed movement and defects in mitochondrial morphology. Finally, we observe a decrease in the velocity and extent of mitochondrial movement in yeast in which actin dynamics are reduced but actin cytoskeletal structure is intact. These results support the idea that the movement of mitochondria in yeast is actin polymerization driven and that this movement requires Arp2/3 complex.
Arp2/3复合物与肌动蛋白聚合驱动的单核细胞增生李斯特菌运动有关。在此，我们发现该复合物的两个亚基Arp2p和Arc15p与分离出的酵母线粒体呈现紧密的、不依赖肌动蛋白的结合。Arp2p与线粒体共定位。与这一结果一致，我们在完整酵母中检测到Arp2p依赖的线粒体周围肌动蛋白云的形成。携带ARP2或ARC15基因突变的细胞显示线粒体运动速度降低、所有定向运动丧失以及线粒体形态缺陷。最后，我们观察到在肌动蛋白动力学降低但肌动蛋白细胞骨架结构完整的酵母中，线粒体运动的速度和范围均下降。这些结果支持了这样一种观点，即酵母中线粒体的运动是由肌动蛋白聚合驱动的，并且这种运动需要Arp2/3复合物。
