Furuse M, Tachibana T, Ohgushi A, Ando R, Yoshimatsu T, Denbow D M
Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 812-8581, Fukuoka, Japan.
Neurosci Lett. 2001 Mar 30;301(2):123-6. doi: 10.1016/s0304-3940(01)01621-4.
Growth hormone releasing factor (GRF) is known to stimulate feeding of rats. Ghrelin, a novel growth hormone (GH)-releasing acylated peptide, was recently isolated from rat stomach. It also stimulates the release of GH from the anterior pituitary through the GH secretagogue receptor (GHS-R) and feeding in the rat. We have investigated the effects of ghrelin and GRF on food intake of the neonatal chick. In Experiment 1, 0, 1.25, 2.5 and 5 microg of ghrelin were administered intracerebroventricularly (i.c.v.) to ad libitum fed birds. In Experiment 2, the effect of (i.c.v.) injection of 0, 1.25, 2.5 and 5 microg of GRF was investigated. Both peptides strongly inhibited food intake of the chick during the 2-h post-injection period. In the third experiment, 0, 0.5, 1 and 2 microg of ghrelin was injected i.c.v. in chicks previously deprived of food for 3 h. Food intake was again inhibited by ghrelin in a dose-dependent manner. These results suggest that the mechanisms for feeding of the neonatal chick through GH release are different from mammals.
生长激素释放因子（GRF）已知可刺激大鼠进食。Ghrelin是一种新发现的可释放生长激素（GH）的酰化肽，最近从大鼠胃中分离出来。它还通过生长激素促分泌素受体（GHS-R）刺激垂体前叶释放GH，并刺激大鼠进食。我们研究了ghrelin和GRF对新生雏鸡食物摄入量的影响。在实验1中，对自由采食的雏鸡脑室内注射（i.c.v.）0、1.25、2.5和5微克的ghrelin。在实验2中，研究了脑室内注射（i.c.v.）0、1.25、2.5和5微克GRF的效果。两种肽在注射后2小时内均强烈抑制雏鸡的食物摄入量。在第三个实验中，对先前禁食3小时的雏鸡脑室内注射0、0.5、1和2微克的ghrelin。Ghrelin再次以剂量依赖的方式抑制食物摄入量。这些结果表明，新生雏鸡通过GH释放进行进食的机制与哺乳动物不同。
