Zeng L, Kong X T, Su J W, Xia T L, Na Y Q, Guo Y L
Institute of Urology, Peking University, Beijing, China.
Asian J Androl. 2001 Mar;3(1):63-6.
To explore the usefulness of proliferating cell nuclear antigen proliferating index (PCNA PI) in the pathological diagnosis and treatment of male infertility.
Testicular biopsy specimen obtained from 48 cases of male infertility and 2 normal controls were fixed and embedded. The sections were stained with anti-PCNA monoclonal antibodies or haematoxylin/eosin. Proliferating index (PI), expressed as the percentage of germ-cell nuclei positively stained with PCNA antibody, was assessed from more than 20 seminiferous tubules or 600 germ-cells.
The infertile patients were divided into 4 groups: Group 1, normal spermatogenesis (14 cases); Group 2, hypospermatogenesis (16 cases); Group 3, germinal arrest (10 cases); Group 4, Sertoli cell only syndrome (8 cases). The PCNA PI of normal control testis was 86.5% (mean value). Group 3 had a significantly lower PCNA PI (29.8%) than normal testis; Group 1 and 2 had similar PIs (82.3% and 82.3%, respectively) as the control testis. PI of the negative control (Group 4) was 0 as no germ-cells were found.
PCNA PI is useful for assessing germ-cell kinetics, especially for pathological diagnosis of germinal arrest which is difficult to differentiate by routine HE staining technique. In germinal arrest, there is a significantly lowered PCNA PI, which is an indication of DNA synthesis deterioration, suggesting the use of therapies be different from those for hypospermatogenesis.
探讨增殖细胞核抗原增殖指数（PCNA PI）在男性不育症病理诊断及治疗中的应用价值。
对48例男性不育患者及2例正常对照者的睾丸活检标本进行固定及包埋。切片用抗PCNA单克隆抗体或苏木精/伊红染色。增殖指数（PI）以PCNA抗体阳性染色的生殖细胞核百分比表示，从20多个生精小管或600个生殖细胞中进行评估。
不育患者分为4组：第1组，生精正常（14例）；第2组，生精低下（16例）；第3组，生精停滞（10例）；第4组，唯支持细胞综合征（8例）。正常对照睾丸的PCNA PI为86.5%（平均值）。第3组的PCNA PI（29.8%）显著低于正常睾丸；第1组和第2组的PI（分别为82.3%和82.3%）与对照睾丸相似。阴性对照（第4组）的PI为0，因为未发现生殖细胞。
PCNA PI有助于评估生殖细胞动力学，尤其是对常规HE染色技术难以鉴别的生精停滞进行病理诊断。在生精停滞中，PCNA PI显著降低，这表明DNA合成恶化，提示其治疗方法应与生精低下不同。
