Filler T J, Bremer C, Peuker E T, Bankert J, Kreft G, Reimer P
Institut für Anatomie, Westfälische Wilhelms-Universität Münster, Vesallusweg 2-4, 48149 Münster.
Radiologe. 2001 Feb;41(2):181-6. doi: 10.1007/s001170050961.
The aim of this study was to analyse pathomorphological findings after treatment with laser induced tumor thermotherapy (LITT) on liver tissue and to correlate the results with magnetic resonance imaging. LITT was performed ex vivo and in vivo using a Neodym-YAG-Laser. Lesions were monitored by MR-thermometry ex vivo and by contrast-enhanced MRI in vivo. After LITT the lesions were examined macroscopically, histologically, and electronmicroscopically. LITT-induced tissue damage was qualitatively evaluated, classified, and quantified by means of digital image analysis. Four different zones of tissue damage were identified within the lesions. Adjacent to the applicator the tissue was completely ablated while more peripheral lesions exhibited only sublethal cell damages seen by EM. In vivo the pattern of tissue injury followed the lobular architecture of the liver tissue. Ultrastructural examination revealed only in areas of minor tissue injury intact sinusoidal patterns. MRI overestimated the diameter of the core zone of complete tissue ablation both ex vivo and to a lesser extent in vivo.
本研究的目的是分析激光诱导肿瘤热疗（LITT）治疗肝脏组织后的病理形态学结果，并将结果与磁共振成像相关联。使用钕钇铝石榴石激光在体外和体内进行LITT。通过体外磁共振热成像和体内对比增强MRI监测病变。LITT后，对病变进行宏观、组织学和电子显微镜检查。通过数字图像分析对LITT诱导的组织损伤进行定性评估、分类和量化。在病变内确定了四个不同的组织损伤区域。靠近施照器的组织被完全消融，而更多外周病变仅表现出电镜下可见的亚致死性细胞损伤。在体内，组织损伤模式遵循肝组织的小叶结构。超微结构检查仅在轻度组织损伤区域发现完整的窦状模式。磁共振成像在体外高估了完全组织消融核心区的直径，在体内高估程度较小。
