Xynos I D, Edgar A J, Buttery L D, Hench L L, Polak J M
Department of Histochemistry, Hammersmith Campus, Imperial College School of Science, Technology and Medicine, London, United Kingdom.
J Biomed Mater Res. 2001 May;55(2):151-7. doi: 10.1002/1097-4636(200105)55:2<151::aid-jbm1001>3.0.co;2-d.
The effect of the ionic products of Bioglass 45S5 dissolution on the gene-expression profile of human osteoblasts was investigated by cDNA microarray analysis of 1,176 genes. Treatment with the ionic products of Bioglass 45S5 dissolution increased the levels of 60 transcripts twofold or more and reduced the levels of five transcripts to one-half or less than in control. Markedly up-regulated genes included RCL, a c-myc responsive growth related gene, cell cycle regulators such as G1/S specific cyclin D1, and apoptosis regulators including calpain and defender against cell death (DAD1). Other significantly up-regulated genes included the cell surface receptors CD44 and integrin beta1, and various extracellular matrix regulators including metalloproteinases-2 and -4 and their inhibitors TIMP-1 and TIMP-2. The identification of differentially expressed genes by cDNA microarray analysis has offered new insights into the mode of action of bioactive glasses and has proven to be an effective tool in evaluating their osteoproductive properties.
通过对1176个基因进行cDNA微阵列分析，研究了生物玻璃45S5溶解离子产物对人成骨细胞基因表达谱的影响。用生物玻璃45S5溶解离子产物处理后，60种转录本的水平增加了两倍或更多，5种转录本的水平降至对照的二分之一或更低。显著上调的基因包括RCL（一种c-myc反应性生长相关基因）、细胞周期调节因子如G1/S特异性细胞周期蛋白D1，以及凋亡调节因子包括钙蛋白酶和抗细胞死亡因子（DAD1）。其他显著上调的基因包括细胞表面受体CD44和整合素β1，以及各种细胞外基质调节因子，包括金属蛋白酶-2和-4及其抑制剂TIMP-1和TIMP-2。通过cDNA微阵列分析鉴定差异表达基因，为生物活性玻璃的作用模式提供了新的见解，并已被证明是评估其骨生成特性的有效工具。
