Singh H, Kumar S, Dewan S, Kumar V L
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, 110 029, New Delhi, India.
J Pharmacol Toxicol Methods. 2000 May-Jun;43(3):219-24. doi: 10.1016/s1056-8719(00)00105-2.
Latex of Calotropis procera was studied for its inflammatory reactions using pedal oedema and air pouch models of inflammation in rats. Subcutaneous injection of aqueous solution (0.1 ml of 1%) of dry latex (DL) into the plantar surface of paw produced significant inflammation. Maximum inflammatory response was obtained 1 h after the injection and was maintained for a further 1 h. The inflammatory response was accompanied by an increase in vascular permeability that reached its maximum within 15 min. Inflammation was also induced in the 6-day-old rat air pouch by injecting a 2.5% solution of DL. The latter model was characterized for the exudate volume and its protein concentration, and wet and dry weights of granuloma. A time-course study indicated that both the exudate volume and the weight of granuloma were at maximum on day 5 after DL injection while the protein concentration peaked on the third day. Further, the two models were also studied for the anti-inflammatory effect of various drugs. It was observed that in the pedal oedema model, phenylbutazone was more effective than prednisolone while almost complete inhibition was produced by mepyramine and cyproheptadine. On the other hand, in the air pouch model, prednisolone was more effective than phenylbutazone in inhibiting the inflammation. Thus, the DL-induced inflammation in different models could be used to evaluate anti-inflammatory drugs.
利用大鼠足跖水肿和气囊炎症模型，对牛角瓜乳胶的炎症反应进行了研究。将干燥乳胶（DL）的水溶液（1%的0.1 ml）皮下注射到大鼠爪的足底表面，可产生明显的炎症。注射后1小时获得最大炎症反应，并持续1小时。炎症反应伴随着血管通透性增加，在15分钟内达到最大值。通过注射2.5%的DL溶液，也可在6日龄大鼠的气囊中诱导炎症。后一种模型以渗出液体积及其蛋白质浓度、肉芽肿的湿重和干重为特征。一项时间进程研究表明，渗出液体积和肉芽肿重量在注射DL后第5天达到最大值，而蛋白质浓度在第3天达到峰值。此外，还研究了这两种模型对各种药物的抗炎作用。观察到在足跖水肿模型中，保泰松比泼尼松龙更有效，而美吡拉敏和赛庚啶几乎可产生完全抑制作用。另一方面，在气囊模型中，泼尼松龙在抑制炎症方面比保泰松更有效。因此，DL在不同模型中诱导的炎症可用于评估抗炎药物。
