Mensah A Y, Donkor P O, Fleischer T C
Department of Pharmacognosy, Faculty of Pharmacy & Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Afr J Tradit Complement Altern Med. 2011;8(2):185-95. doi: 10.4314/ajtcam.v8i2.63207. Epub 2010 Dec 30.
The present study determined the anti-inflammatory activity of Wissadula amplissima var rostrata (Schum. & Thonn.), and calculated the total phenolic content and total antioxidant capacity of the plant in an attempt to justify the traditional uses of the plant in the Ashanti region of Ghana for the management of spider,wasps and bee stings. Powdered dried leaves of Wissadula amplissima were Soxhlet extracted with Petroleum Ether (PWA, yield: 1.46% (w)/(w)); Chloroform (CWA, yield: 1.18% (w)/(w)) and Methanol (MWA, yield: 3.39% (w)/(w)). These fractions were tested for anti-inflammatory activity using carrageenan-induced foot edema in 7 day old chicks. The effect before the induction of inflammation (pre-emptive protocol) paradigm was used for the assessment. Oral administration of PWA, CWA and MWA (30 - 300 mg/kg) dose dependently reduced edema with maximal effects of 68.25±2.03%, 77.83±0.81% and 62.21±2.61% respectively. Similarly the NSAID, Diclofenac (10 - 100 mg/Kg, i.p) and the steroidal anti-inflammatory drug dexamethasone (0.3 - 3 mg/Kg, i.p) used as positive controls, dose-dependently inhibited the edema with maximal effect of 87.96±1.11% and 67.47±3.51% respectively. The potencies exhibited by all three extracts were comparable to that shown by Diclofenac but higher than that of Dexamethasone. Phenols were detected in all three extracts with the highest concentration in the MWA. The extracts also scavenged DPPH with EC(50) values of 0.9784, 0.9096 and 0.2767 for PWA, CWA, MWA respectively. The results of this study give scientific credence to the local use of Wissadula amplissima to modulate inflammation induced by stings of animals.
本研究测定了长喙假商陆（Wissadula amplissima var rostrata (Schum. & Thonn.)）的抗炎活性，并计算了该植物的总酚含量和总抗氧化能力，以验证加纳阿散蒂地区将该植物用于治疗蜘蛛、黄蜂和蜜蜂蜇伤的传统用途。用石油醚（PWA，产率：1.46%（w）/（w））、氯仿（CWA，产率：1.18%（w）/（w））和甲醇（MWA，产率：3.39%（w）/（w））对长喙假商陆的干燥叶粉末进行索氏提取。使用角叉菜胶诱导7日龄雏鸡足部水肿的方法对这些提取物进行抗炎活性测试。采用炎症诱导前（先发制人方案）范式进行评估。口服PWA、CWA和MWA（30 - 300 mg/kg）剂量依赖性地减轻水肿，最大效应分别为68.25±2.03%、77.83±0.81%和62.21±2.61%。同样，用作阳性对照的非甾体抗炎药双氯芬酸（10 - 100 mg/Kg，腹腔注射）和甾体抗炎药地塞米松（0.3 - 3 mg/Kg，腹腔注射）剂量依赖性地抑制水肿，最大效应分别为87.96±1.11%和67.47±3.51%。三种提取物表现出的效力与双氯芬酸相当，但高于地塞米松。在所有三种提取物中均检测到酚类物质，其中MWA中的浓度最高。这些提取物还清除DPPH，PWA、CWA、MWA的EC(50)值分别为0.9784、0.9096和0.2767。本研究结果为长喙假商陆在当地用于调节动物蜇伤引起的炎症提供了科学依据。
