Arya Soneera, Kumar Vijay L
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India.
Inflammopharmacology. 2004;12(4):391-8. doi: 10.1163/1568560043696254.
Interleukin-1beta (IL-1beta), a pro-inflammatory cytokine, has been reported to exhibit anti-inflammatory properties in the carrageenan-induced paw oedema model. In the present study, we have evaluated the anti-inflammatory activity of IL-1beta against inflammation induced by local administration of the methanol extract of dried latex of Calotropis procera (MeDL) and compared it with that against carrageenan. The anti-inflammatory activity of standard anti-inflammatory drugs, phenylbutazone (PBZ) and dexamethasone (DEX), was also evaluated against both inflammagens. Injection of an aqueous solution of dried latex and MeDL into the sub-plantar surface of the rat paw produced intense inflammation with a peak response occurring within 2 h, while the peak inflammatory response with carrageenan was obtained at 3 h. Subcutaneous injection of IL-1beta was found to be more effective against the inflammatory response elicited by carrageenan (70% inhibition) as compared to MeDL (50% inhibition) at 20microg/kg dose. On the other hand, PBZ effectively inhibited the inflammatory response elicited by both MeDL and carrageenan, while DEX was more effective against carrageenan. Thus, our study indicates that the difference in the anti-inflammatory effect of IL-1beta against latex of C. procera extract and carrageenan is due to the release of different mediators released by these inflammagens.
白细胞介素-1β（IL-1β）是一种促炎细胞因子，据报道在角叉菜胶诱导的爪肿胀模型中具有抗炎特性。在本研究中，我们评估了IL-1β对由本地施用牛角瓜干燥乳胶甲醇提取物（MeDL）诱导的炎症的抗炎活性，并将其与对角叉菜胶的抗炎活性进行比较。还评估了标准抗炎药物保泰松（PBZ）和地塞米松（DEX）对两种炎症介质的抗炎活性。将干燥乳胶和MeDL的水溶液注射到大鼠爪的足底表面下会产生强烈的炎症，在2小时内出现峰值反应，而角叉菜胶的峰值炎症反应在3小时时出现。皮下注射IL-1β被发现与MeDL（50%抑制）相比，在20μg/kg剂量下对角叉菜胶引发的炎症反应更有效（70%抑制）。另一方面，PBZ有效抑制了MeDL和角叉菜胶引发的炎症反应，而DEX对角叉菜胶更有效。因此，我们的研究表明，IL-1β对牛角瓜提取物乳胶和角叉菜胶的抗炎作用差异是由于这些炎症介质释放的不同介质所致。
