Shivkar Yatin M, Kumar Vijay L
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India.
Mediators Inflamm. 2003 Oct;12(5):299-302. doi: 10.1080/096293503310001619708.
Calotropis procera is known to produce contact dermatitis and the latex of this plant produces intense inflammation when injected locally. However, the precise mode of its pro-inflammatory effect is not known. In present study we have pharmacologically characterized the inflammation induced by latex of C. procera in a rat paw edema model and determined the role of histamine in latex-induced inflammation.
Inflammation was induced in the hind paw of rats by injecting different doses of dried latex (DL) of C. procera. The inhibitory effect of phenylbutazone, dexamethasone, celecoxib, cyproheptadine, chlorpheniramine and compound 48/80 on edema volume was evaluated and compared with that against carrageenan. The histamine content of DL was measured fluorometrically.
DL produced dose-dependent inflammation of the rat paw. Cyproheptadine and chlorpheniramine effectively inhibited DL-induced inflammation (90%; p < 0.01), while anti-inflammatory drugs phenylbutazone, dexamethasone and celecoxib were more effective against carrageenan-induced inflammation. Depletion of mast cell histamine by compound 48/80 produced a significant decrease in DL-induced inflammation as compared with carrageenan (500% versus 25%). DL was also found to contain about 6 microg/g of histamine.
Thus, our study shows that the biogenic amines play a significant role in C. procera latex-induced inflammation and antihistaminic drugs could be effectively used to inhibit inflammatory response elicited by exposure to latex.
已知牛角瓜会引起接触性皮炎，该植物的乳胶局部注射时会引发强烈炎症。然而，其促炎作用的确切方式尚不清楚。在本研究中，我们在大鼠足爪水肿模型中对牛角瓜乳胶诱导的炎症进行了药理学特征分析，并确定了组胺在乳胶诱导炎症中的作用。
通过注射不同剂量的牛角瓜干燥乳胶（DL）诱导大鼠后爪炎症。评估了保泰松、地塞米松、塞来昔布、赛庚啶、氯苯那敏和化合物48/80对水肿体积的抑制作用，并与对角叉菜胶的抑制作用进行比较。采用荧光法测定DL中的组胺含量。
DL可引起大鼠足爪剂量依赖性炎症。赛庚啶和氯苯那敏有效抑制了DL诱导的炎症（90%；p<0.01），而抗炎药保泰松、地塞米松和塞来昔布对角叉菜胶诱导的炎症更有效。与角叉菜胶相比，化合物48/80使肥大细胞组胺耗竭后，DL诱导的炎症显著降低（500%对25%）。还发现DL中含有约6微克/克的组胺。
因此，我们的研究表明，生物胺在牛角瓜乳胶诱导的炎症中起重要作用，抗组胺药物可有效用于抑制接触乳胶引起的炎症反应。
