Hunt H D, Lupiani B, Miller M M, Gimeno I, Lee L F, Parcells M S
U.S. Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, 3606 East Mount Hope Road, East Lansing, Michigan 48863, USA.
Virology. 2001 Mar 30;282(1):198-205. doi: 10.1006/viro.2000.0797.
Infection of chicken cells with three Marek's disease virus (MDV) serotypes interferes with expression of the major histocompatibility complex (MHC or B complex) class I (BF) glycoproteins. BF surface expression is blocked after infection of OU2 cells with MDV serotypes 1, 2, and 3. MDV-induced T-cell tumors suffer a nearly complete loss of cell surface BF upon virus reactivation with 5-bromo-2'-deoxyuridine (BUdR). The recombinant virus (RB1BUS2gfpDelta) transforming the MDCC-UA04 cell line expresses green fluorescent protein (GFP) during the immediate early phase of viral gene expression. Of the UA04 cells induced to express the immediate early GFP, approximately 60% have reduced levels of BF expression. All of the reactivated UA04 and MSB1 tumor cells expressing the major early viral protein pp38 display reduced levels of BF. Thus, BF down-regulation begins in the immediate early phase and is complete by the early phase of viral gene expression. The intracellular pool of BF is not appreciably affected, indicating that the likely mechanism is a block in BF transport and not the result of transcriptional or translational regulation.
用三种马立克氏病病毒（MDV）血清型感染鸡细胞会干扰主要组织相容性复合体（MHC或B复合体）I类（BF）糖蛋白的表达。用MDV血清型1、2和3感染OU2细胞后，BF的表面表达被阻断。在用5-溴-2'-脱氧尿苷（BUdR）重新激活病毒后，MDV诱导的T细胞肿瘤细胞表面BF几乎完全丧失。转化MDCC-UA04细胞系的重组病毒（RB1BUS2gfpDelta）在病毒基因表达的即刻早期阶段表达绿色荧光蛋白（GFP）。在被诱导表达即刻早期GFP的UA04细胞中，约60%的细胞BF表达水平降低。所有重新激活的表达主要早期病毒蛋白pp38的UA04和MSB1肿瘤细胞BF水平均降低。因此，BF下调始于即刻早期阶段，并在病毒基因表达的早期阶段完成。BF的细胞内池没有受到明显影响，这表明可能的机制是BF转运受阻，而不是转录或翻译调控的结果。
