来自黑桑的一种甘露糖特异性杰克豆凝集素相关凝集素的晶体呈准同形孪晶。

The crystals of a mannose-specific jacalin-related lectin from Morus nigra are merohedrally twinned.

作者信息

Rabijns A, Verboven C, Novoa de Armas H, Van Damme E J, Peumans W J, De Ranter C J

机构信息

Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K. U. Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.

出版信息

Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):609-11. doi: 10.1107/s0907444901002104.

DOI:10.1107/s0907444901002104

PMID:11264597

Abstract

MornigaM, a lectin from Morus nigra, belongs to the mannose-binding subgroup of the family of jacalin-related plant lectins. It was crystallized in the P6(5) space group, with unit-cell parameters a = b = 110.74, c = 159.28 A. The partially merohedrally twinned crystals could be detwinned and a subsequent molecular-replacement solution could be found using the coordinates of jacalin. Preliminary analysis clearly shows the tetrameric assembly of this protein. Furthermore, data from MornigaM crystals soaked in a mannose solution were collected.

摘要

黑桑凝集素MornigaM属于jacalin相关植物凝集素家族的甘露糖结合亚组。它在P6(5)空间群中结晶，晶胞参数a = b = 110.74，c = 159.28 Å。部分merohedrally孪晶的晶体可以解孪晶，随后使用jacalin的坐标可以找到分子置换解。初步分析清楚地显示了该蛋白质的四聚体组装。此外，还收集了浸泡在甘露糖溶液中的MornigaM晶体的数据。