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通过小双链RNA的序列分析追踪微小隐孢子虫

Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA.

作者信息

Xiao L, Limor J, Bern C, Lal A A

机构信息

Division of Parasitic Diseases, Centers for Disease Control and Prevention, Mail Stop F12, Atlanta, GA 30333, USA.

出版信息

Emerg Infect Dis. 2001 Jan-Feb;7(1):141-5. doi: 10.3201/eid0701.010121.

DOI:10.3201/eid0701.010121
PMID:11266306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2631692/
Abstract

We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique may be useful for tracking Cryptosporidium infection sources.

摘要

我们对来自23头小牛和38名人类的微小隐孢子虫分离株的双链病毒样小RNA的173个核苷酸片段进行了测序。在17个位点检测到序列多样性。来自同一疫情爆发的分离株具有相同的双链RNA序列,这表明该技术可能有助于追踪隐孢子虫感染源。

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本文引用的文献

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Presence of double-stranded RNAs in human and calf isolates of Cryptosporidium parvum.人源和牛源微小隐孢子虫分离株中双链RNA的存在情况。
J Parasitol. 2000 Apr;86(2):275-82. doi: 10.1645/0022-3395(2000)086[0275:PODSRI]2.0.CO;2.
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An outbreak of cryptosporidiosis linked to a foodhandler.一起与食品从业人员相关的隐孢子虫病暴发。
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Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis.对218例被诊断为散发性隐孢子虫病的腹泻患者的隐孢子虫菌株进行基因特征分析。
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