在同时含有恶性大鼠乳腺癌肉瘤（Walker - 256）细胞和正常成年大鼠肝脏成纤维细胞的培养物中，用同型半胱氨酸替代甲硫氨酸的效果。
Effect of methionine replacement by homocystine in cultures containing both malignant rat breast carcinosarcoma (Walker-256) cells and normal adult rat liver fibroblasts.
作者信息
Halpern B C, Ezzell R, Hardy D N, Clark B R, Ashe H, Halpern R M, Smith R A
出版信息
In Vitro. 1975 Jan-Feb;11(1):14-9. doi: 10.1007/BF02615317.
When malignant W-256 rat breast carcinosarcoma cells are mixed with an equal number of normal adult rat liver fibroblasts and allowed to grow in a medium containing sufficient L-methionine and an excess of vitamin B12 and of folic acid, the malignant cells outgrow the normal cells, and within 2 weeks the tissue culture flasks contain only neoplastic cells. However, when ample DL-homocystine or homocysteine replaces methionine in the medium containing the same amount of vitamin B12 and folic acid, and seeded with the same type and number of malignant and normal cells, the malignant cells die and the normal cells thrive. Substantiating this conclusion are the results of injections into rats of comparable numbers of cells from each group after 3 weeks of growth in tissue culture. Fatal malignancies are produced by the homocystein-cultivated cells.
当恶性W - 256大鼠乳腺癌肉瘤细胞与等量的正常成年大鼠肝脏成纤维细胞混合，并在含有足够L - 蛋氨酸、过量维生素B12和叶酸的培养基中培养时，恶性细胞的生长超过正常细胞，2周内组织培养瓶中仅含有肿瘤细胞。然而，当在含有相同量维生素B12和叶酸的培养基中，用充足的DL - 高胱氨酸或高半胱氨酸替代蛋氨酸，并接种相同类型和数量的恶性和正常细胞时，恶性细胞死亡而正常细胞生长旺盛。在组织培养中生长3周后，将每组数量相当的细胞注射到大鼠体内的结果证实了这一结论。高半胱氨酸培养的细胞会产生致命的恶性肿瘤。
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