College of Chemistry, Sichuan University, Chengdu, PR China.
Talanta. 2011 Sep 15;85(3):1279-84. doi: 10.1016/j.talanta.2011.06.007. Epub 2011 Jun 12.
A simple and sensitive method has been developed for simultaneous analysis of three catecholamines: dopamine (DA), epinephrine (EP) and norepinephrine (NE) in urine by capillary electrophoresis (CE) coupled with in-column fiber-optic light-emitting diode-induced fluorescence detection (ICFO-LED-IFD). Fluorescein isothiocyanate was used as the fluorescence tagged reagent for derivatization of DA, EP and NE. The CE conditions for separation of these catecholamines were systematically investigated. It was found that catecholamines could be more effectively separated by adding β-cyclodextin (β-CD) and acetonitrile (ACN) to a background electrolyte (BGE) of sodium borate. The migration times are 10.61, 10.83 and 11.14 min for DA, EP and NE, respectively and the catecholamines are completely separated within 11.5 min under the optimal condition of a BGE containing 10% v/v ACN, 20 mM β-CD and 20 mM sodium borate (pH 9.5), and an applied voltage of 13 kV. The relative standard deviations of migration time and peak area for these catecholamines are less than 0.16 and 2.0%, respectively. The limit of quantifications (LOQs) for DA, EP and NE are 3.5, 1.0 and 3.1 nM whereas the limit of detections (LODs) for DA, EP and NE are 1.0, 0.3 and 0.9 nM, respectively. Our proposed CE method provides low LOQ and LOD values. This CE-ICFO-LED-IFD methodology has been successfully applied to analyze catecholamines in human urine samples with good accuracy and satisfactory recovery.
已开发出一种简单灵敏的方法，用于通过毛细管电泳（CE）与柱内光纤发光二极管诱导荧光检测（ICFO-LED-IFD）联用，同时分析尿液中的三种儿茶酚胺：多巴胺（DA）、肾上腺素（EP）和去甲肾上腺素（NE）。荧光素异硫氰酸酯被用作衍生 DA、EP 和 NE 的荧光标记试剂。系统地研究了这些儿茶酚胺的 CE 分离条件。结果发现，通过向硼酸钠背景电解质（BGE）中添加β-环糊精（β-CD）和乙腈（ACN），可以更有效地分离儿茶酚胺。DA、EP 和 NE 的迁移时间分别为 10.61、10.83 和 11.14 min，在最佳条件下，BGE 中含有 10% v/v ACN、20 mM β-CD 和 20 mM 硼酸钠（pH 9.5），施加 13 kV 电压，儿茶酚胺可在 11.5 min 内完全分离。这些儿茶酚胺的迁移时间和峰面积的相对标准偏差均小于 0.16%和 2.0%。DA、EP 和 NE 的定量限（LOQs）分别为 3.5、1.0 和 3.1 nM，而 DA、EP 和 NE 的检测限（LODs）分别为 1.0、0.3 和 0.9 nM。我们提出的 CE 方法提供了低的 LOQ 和 LOD 值。该 CE-ICFO-LED-IFD 方法已成功应用于分析人尿液样品中的儿茶酚胺，具有良好的准确性和令人满意的回收率。
