Vasconcelos V M, Pereira E
Faculdade de Ciências, Departamento de Zoologia e Antropologia, Praça Gomes, Teixeira, 4050 Porto, Portugal.
Water Res. 2001 Apr;35(5):1354-7. doi: 10.1016/s0043-1354(00)00512-1.
Cyanobacteria are common in eutrophic natural waters. Being favoured by warm, stable and nutrient-enriched waters they may constitute an important part of the phytoplankton community in Wastewater Treatment Plants (WWTP). The phytoplankton communities of two ponds (facultative and maturation) of the WWTP of Esmoriz (North Portugal) were studied, with particular importance given to cyanobacteria. Mouse bioassays were performed with cyanobacteria samples during some of the blooms and ELISA assays specific for hepatotoxic microcystins were carried out. During the study period (January-July 1999) cyanobacteria were frequently dominant in the ponds ranging from 15.2 to 99.8% of the total phytoplankton density. The main species were Planktothrix mougeotii, Microcystis aeruginosa and Pseudanabaena mucicola. Mouse bioassays were performed during Oscillatoria bloom period but the results were negative, in spite of the high cyanobacteria biomass. ELISA assays were performed for both ponds but only in the maturation pond positive values were found. Microcystin concentrations (as MCYST-LR equivalents) varied from 2.3 to 56.0 micrograms/l on the margin of the pond and between 1.7 and 4.6 micrograms/l in the outflow of this pond. These values indicate that WWTP may be a source of contamination of water bodies with cyanobacteria toxins.
蓝藻在富营养化的天然水体中很常见。由于它们偏爱温暖、稳定且营养丰富的水体，所以可能构成污水处理厂（WWTP）浮游植物群落的重要组成部分。对位于葡萄牙北部埃斯莫里兹的污水处理厂的两个池塘（兼性塘和熟化塘）的浮游植物群落进行了研究，其中特别关注了蓝藻。在某些水华期间，对蓝藻样本进行了小鼠生物测定，并开展了针对肝毒性微囊藻毒素的酶联免疫吸附测定（ELISA）。在研究期间（1999年1月至7月），蓝藻在池塘中经常占据主导地位，占浮游植物总密度的15.2%至99.8%。主要物种有穆氏席藻、铜绿微囊藻和粘质伪鱼腥藻。在颤藻水华期间进行了小鼠生物测定，但尽管蓝藻生物量很高，结果却是阴性。对两个池塘都进行了ELISA测定，但仅在熟化塘中发现了阳性值。池塘边缘微囊藻毒素浓度（以微囊藻毒素-LR当量计）在2.3至56.0微克/升之间，该池塘流出水中的浓度在1.7至4.6微克/升之间。这些值表明污水处理厂可能是水体受蓝藻毒素污染的一个来源。
