Gillespie J W, Ahram M, Best C J, Swalwell J I, Krizman D B, Petricoin E F, Liotta L A, Emmert-Buck M R
Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892, USA.
Cancer J. 2001 Jan-Feb;7(1):32-9.
Tissue microdissection is a laboratory method that is used to procure specific cells or cell populations from a histology slide under direct microscopic visualization. The recovered cells can be studied with a variety of DNA, messenger RNA, and protein analysis methods, including new high-throughput gene expression and proteomics technologies. This approach is permitting investigators to comprehensivelyexamine the molecular anatomy of cells in tissue sections forthe first time. This article reviews the development and evolution of tissue microdissection techniques, summarizes examples of research studies, and discusses related challenges that the research community must address. Additional information and complete laboratory protocols are available on a website at http://cgap-mf.nih.gov/.
组织显微切割是一种实验室方法，用于在直接显微镜观察下从组织学载玻片上获取特定细胞或细胞群体。回收的细胞可以通过多种DNA、信使RNA和蛋白质分析方法进行研究，包括新的高通量基因表达和蛋白质组学技术。这种方法首次使研究人员能够全面检查组织切片中细胞的分子解剖结构。本文回顾了组织显微切割技术的发展和演变，总结了研究实例，并讨论了研究界必须应对的相关挑战。更多信息和完整的实验室方案可在网站http://cgap-mf.nih.gov/上获取。
