Sobti R C, Kochar J, Singh K, Bhasin D, Capalash N
Department of Biotechnology, Panjab University, Chandigarh, India.
Mol Cell Biochem. 2001 Jan;217(1-2):51-6. doi: 10.1023/a:1007224001047.
The present study was initiated with the objective of finding out the role of possible factors in the etiology of gastrointestinal tract cancers. HPV-DNA was detected in 62.5% (25/40) of the patients by PCR. Telomerase activity as shown by TRAP-ELISA assay was detected in 82.5% (33/40) of the tumor samples and absent in 85.7% (30/35) of the normal samples taken from the same patients. As many as 53.6% (15/28) of the invasive cases were positive both for telomerase activity and for HPV, while 39.3% (11/28) of them, although indicating telomerase expression, showed no signal for HPV. This suggests that activation of telomerase could be by a pathway independent of HPV activation, although both parameters could act as diagnostic and prognostic markers for gastrointestinal tract cancers.
本研究旨在探究可能的因素在胃肠道癌症病因学中的作用。通过聚合酶链反应（PCR）在62.5%（25/40）的患者中检测到了人乳头瘤病毒DNA（HPV-DNA）。端粒酶活性通过端粒重复序列扩增法-酶联免疫吸附测定（TRAP-ELISA）检测，在82.5%（33/40）的肿瘤样本中被检测到，而在取自同一患者的85.7%（30/35）的正常样本中未检测到。多达53.6%（15/28）的浸润性病例端粒酶活性和HPV均呈阳性，而其中39.3%（11/28）的病例尽管显示端粒酶表达，但未检测到HPV信号。这表明端粒酶的激活可能通过一条独立于HPV激活的途径，尽管这两个参数都可作为胃肠道癌症的诊断和预后标志物。
